Westphal C, Frösch D
J Ultrastruct Res. 1984 Sep;88(3):282-6. doi: 10.1016/s0022-5320(84)90126-6.
It is shown that electron-phase-contrast images of unstained, melamine-embedded membranes, muscles and collagen fibers favorably compare with images recorded from corresponding preparations which have in addition been stained with heavy metals, with regard to both contrast and resolution of structural detail.
结果表明,对于未染色的、三聚氰胺包埋的膜、肌肉和胶原纤维的电子相衬图像,在结构细节的对比度和分辨率方面,与从相应的、另外还用重金属染色的标本所记录的图像相比毫不逊色。