Frösch D, Westphal C
Sektion Elektronenmikroskopie, Universität Ulm, ULM/Donau, F.R.G.
Electron Microsc Rev. 1989;2(2):231-55. doi: 10.1016/0892-0354(89)90002-6.
Melamine resins are derived from the heterocyclic compound triaminotriazine, C3H6N6. Similar to proteins in structure and reactivity, water-soluble melamine resins can be used as water-embedding media for electron microscopy (Bachhuber and Frösch, 1983). The idea behind this approach was to study some of the artefacts of traditional embedding techniques and to work out conditions to eliminate as far as possible denaturing of proteins and extraction of lipids. Sectioned cells and tissues processed in the melamine resin Nanoplast show remarkable preservation of ultrastructure. Because they can be sectioned extremely thinly, melamine resins are particularly suitable for dark-field and electron spectroscopic imaging of unstained molecular suspensions providing in this way an unusually clear reproduction of ultrastructural detail such as the helical structure of isolated unstained double-strand DNA molecules (Frösch et al., 1987b). In 1988, the melamine resin Nanostrat was introduced as an EM-compatible prolific substrate foil for cell culture (Westphal et al., 1988). Cells or bacteria cultivated on this material can be processed for various kinds of follow-up techniques like TEM, SEM, vertical sectioning and immunocytochemistry.
三聚氰胺树脂由杂环化合物三氨基三嗪（C3H6N6）衍生而来。三聚氰胺树脂在结构和反应性上与蛋白质相似，其水溶性形式可用作电子显微镜的水包埋介质（巴赫胡伯和弗勒施，1983年）。这种方法背后的理念是研究传统包埋技术中的一些假象，并找出尽可能消除蛋白质变性和脂质提取的条件。用三聚氰胺树脂Nanoplast处理的细胞和组织切片显示出超微结构的显著保存。由于三聚氰胺树脂可以切得极薄，因此特别适合对未染色的分子悬浮液进行暗场和电子光谱成像，从而以这种方式异常清晰地再现超微结构细节，例如未染色的分离双链DNA分子的螺旋结构（弗勒施等人，1987b）。1988年，三聚氰胺树脂Nanostrat作为一种与电子显微镜兼容的细胞培养多用途基质箔被引入（韦斯特法尔等人，1988年）。在这种材料上培养的细胞或细菌可用于各种后续技术，如透射电子显微镜、扫描电子显微镜、垂直切片和免疫细胞化学。
