Siffert W, Fox G, Mückenhoff K, Scheid P
FEBS Lett. 1984 Jul 9;172(2):272-4. doi: 10.1016/0014-5793(84)81139-4.
We have investigated the release of protons from thrombin-stimulated platelets. Addition of thrombin to suspensions of washed platelets resulted in fast liberation of H+. In the presence of 0.1 mM amiloride, a potent inhibitor of the Na+/H+ transport system, the amount of protons liberated was decreased by about 50%, and was further reduced to about 15% by 1 mM amiloride. Similar inhibition of H+ release was observed after Na+ in the incubating medium had been replaced by choline. We conclude that one of the earliest events in thrombin-stimulated platelets consists of the activation of an Na+/H+ countertransport, which leads to an increase in intracellular pH.
我们研究了凝血酶刺激的血小板释放质子的情况。向洗涤过的血小板悬液中加入凝血酶会导致H⁺快速释放。在存在0.1 mM氨氯吡咪（一种Na⁺/H⁺转运系统的强效抑制剂）的情况下，释放的质子数减少了约50%，而1 mM氨氯吡咪可使其进一步降至约15%。在用胆碱取代孵育培养基中的Na⁺后，观察到了类似的对H⁺释放的抑制作用。我们得出结论，凝血酶刺激的血小板中最早发生的事件之一是Na⁺/H⁺逆向转运的激活，这会导致细胞内pH值升高。
