凝血酶和佛波酯刺激下人血小板中Na+/H+交换的激活
Activation of Na+/H+ exchange in human platelets stimulated by thrombin and a phorbol ester.
作者信息
Siffert W, Siffert G, Scheid P
出版信息
Biochem J. 1987 Jan 1;241(1):301-3. doi: 10.1042/bj2410301.
Abstract
We have investigated changes in cytoplasmic pH (pHi) in activated human platelets, using the fluorescent probe 2,7-biscarboxyethyl-5(6)-carboxyfluorescein. Stimulation of platelets by thrombin or 12-O-tetradecanoylphorbol 13-acetate increased pHi by about 0.11 pH unit above the resting value. This increase in pHi depended on the presence of external Na+ and was inhibited by ethylisopropylamiloride. The data suggest that protein kinase C mediates Na+/H+ exchange in human platelets.
摘要
我们使用荧光探针2,7-双（羧乙基）-5（6）-羧基荧光素，研究了活化的人血小板中细胞质pH（pHi）的变化。凝血酶或12-O-十四烷酰佛波醇13-乙酸酯刺激血小板后，pHi比静息值升高约0.11个pH单位。pHi的这种升高依赖于细胞外Na+的存在，并被乙基异丙基amiloride抑制。数据表明蛋白激酶C介导人血小板中的Na+/H+交换。
相似文献
1
Activation of Na+/H+ exchange in human platelets stimulated by thrombin and a phorbol ester.凝血酶和佛波酯刺激下人血小板中Na+/H+交换的激活
Biochem J. 1987 Jan 1;241(1):301-3. doi: 10.1042/bj2410301.
2
Ca2+ mobilization can occur independent of acceleration of Na+/H+ exchange in thrombin-stimulated human platelets.在凝血酶刺激的人血小板中，钙离子动员可独立于钠氢交换加速而发生。
J Biol Chem. 1988 Jul 15;263(20):9635-9.
3
4
Na+/H+ exchange in PAF-stimulated platelets.血小板活化因子刺激的血小板中的钠/氢交换
J Lipid Mediat. 1989 Sep-Oct;1(5):257-72.
5
Cytoplasmic pH dependency of Na+/H+ exchange in human platelets activated with thrombin, arachidonic acid, A23187 and TPA.凝血酶、花生四烯酸、A23187和佛波酯激活的人血小板中Na+/H+交换的细胞质pH依赖性
Thromb Res. 1989 Sep 15;55(6):683-93. doi: 10.1016/0049-3848(89)90299-5.
6
7
Thrombin-induced changes of intracellular [Ca2+] and pH in human platelets. Cytoplasmic alkalinization is not a prerequisite for calcium mobilization.凝血酶诱导的人血小板细胞内[Ca2+]和pH值的变化。细胞质碱化并非钙动员的先决条件。
Biochim Biophys Acta. 1988 Mar 3;938(3):497-500. doi: 10.1016/0005-2736(88)90149-6.
8
Further characterization of the mechanisms mediating the rise in cytosolic free Na+ in thrombin-stimulated platelets. Evidence for inhibition of the Na+,K(+)-ATPase and for Na+ entry via a Ca2+ influx pathway.凝血酶刺激的血小板中细胞质游离钠离子浓度升高的介导机制的进一步特征。关于抑制钠钾ATP酶以及通过钙离子内流途径使钠离子进入的证据。
J Biol Chem. 1991 Jul 15;266(20):13153-60.
9
Extracellular Na+, but not Na+/H+ exchange, is necessary for receptor-mediated arachidonate release in platelets.细胞外钠离子而非钠氢交换，是血小板中受体介导的花生四烯酸释放所必需的。
Biochem J. 1990 Jan 1;265(1):155-60. doi: 10.1042/bj2650155.
10
Inhibition of Na+/H+ exchange reduces Ca2+ mobilization without affecting the initial cleavage of phosphatidylinositol 4,5-bisphosphate in thrombin-stimulated platelets.抑制Na+/H+交换可减少Ca2+动员，而不影响凝血酶刺激的血小板中磷脂酰肌醇4,5-二磷酸的初始裂解。
FEBS Lett. 1987 Feb 9;212(1):123-6. doi: 10.1016/0014-5793(87)81569-7.
引用本文的文献
1
Protein kinases in amphibian ectoderm induced for neural differentiation.诱导神经分化的两栖类外胚层中的蛋白激酶。
Rouxs Arch Dev Biol. 1988 Oct;197(6):339-344. doi: 10.1007/BF00375953.
2
Involvement of the Na+/H+ exchanger in membrane phosphatidylserine exposure during human platelet activation.钠/氢交换体在人血小板激活过程中膜磷脂酰丝氨酸暴露中的作用。
Biochim Biophys Acta. 2006 Feb;1761(2):195-204. doi: 10.1016/j.bbalip.2005.12.008. Epub 2006 Jan 17.
3
Sodium-hydrogen exchange and platelet function.钠氢交换与血小板功能。
J Thromb Thrombolysis. 1999 Jul;8(1):15-24. doi: 10.1023/a:1008986329267.
4
Arachidonic acid activates a proton conductance pathway and the Na+/H+ exchanger in platelets.花生四烯酸激活血小板中的质子传导途径和钠/氢交换体。
Biochem J. 1996 Oct 15;319 ( Pt 2)(Pt 2):567-74. doi: 10.1042/bj3190567.
5
Na+/H+ exchange in human lymphocytes and platelets in chronic and subacute metabolic acidosis.慢性和亚急性代谢性酸中毒时人淋巴细胞和血小板中的钠/氢交换
J Clin Invest. 1993 Aug;92(2):858-65. doi: 10.1172/JCI116660.
6
Intracellular calcium mobilization and activation of the Na+/H+ exchanger in platelets.血小板内的钙动员及钠/氢交换体的激活
Biochem J. 1993 Mar 1;290 ( Pt 2)(Pt 2):617-22. doi: 10.1042/bj2900617.
7
Dephosphorylation of cofilin in stimulated platelets: roles for a GTP-binding protein and Ca2+.刺激的血小板中丝切蛋白的去磷酸化：一种GTP结合蛋白和Ca2+的作用。
Biochem J. 1994 Jul 1;301 ( Pt 1)(Pt 1):41-7. doi: 10.1042/bj3010041.
8
Phosphorylation-dependent and -independent pathways of platelet aggregation.血小板聚集的磷酸化依赖性和非依赖性途径。
Biochem J. 1989 Mar 1;258(2):479-85. doi: 10.1042/bj2580479.
9
Activation of Na+/H+ exchange and Ca2+ mobilization start simultaneously in thrombin-stimulated platelets. Evidence that platelet shape change disturbs early rises of BCECF fluorescence which causes an underestimation of actual cytosolic alkalinization.在凝血酶刺激的血小板中，Na⁺/H⁺交换的激活和Ca²⁺动员同时开始。有证据表明，血小板形状改变会干扰BCECF荧光的早期升高，从而导致对实际胞质碱化的低估。
Biochem J. 1989 Mar 1;258(2):521-7. doi: 10.1042/bj2580521.
10
Thrombin-induced cytosolic alkalinization in human platelets occurs without an apparent involvement of HCO3-/Cl- exchange.凝血酶诱导的人血小板胞质碱化发生时，碳酸氢根离子/氯离子交换似乎未参与其中。
Pflugers Arch. 1990 Apr;416(1-2):68-73. doi: 10.1007/BF00370224.
本文引用的文献
1
Movement of sodium into human platelets.
Biochim Biophys Acta. 1980 Aug 4;600(2):448-55. doi: 10.1016/0005-2736(80)90447-2.
2
Changes in cytoplasmic pH and in membrane potential in thrombin-stimulated human platelets.凝血酶刺激的人血小板中细胞质pH值和膜电位的变化。
Eur J Biochem. 1981 Nov;120(2):295-302. doi: 10.1111/j.1432-1033.1981.tb05703.x.
3
Cytoplasmic pH and free Mg2+ in lymphocytes.淋巴细胞中的细胞质pH值和游离镁离子
J Cell Biol. 1982 Oct;95(1):189-96. doi: 10.1083/jcb.95.1.189.
4
Synergistic functions of protein phosphorylation and calcium mobilization in platelet activation.蛋白质磷酸化与钙动员在血小板激活中的协同作用。
J Biol Chem. 1983 Jun 10;258(11):6701-4.
5
Metabolic regulation via intracellular pH.通过细胞内pH值进行的代谢调节
Am J Physiol. 1984 Apr;246(4 Pt 2):R409-38. doi: 10.1152/ajpregu.1984.246.4.R409.
6
Thrombin-induced phosphodiesteratic cleavage of phosphatidylinositol bisphosphate in human platelets.凝血酶诱导人血小板中磷脂酰肌醇二磷酸的磷酸二酯酶裂解
J Biol Chem. 1983 Feb 25;258(4):2076-8.
7
The role of protein kinase C in cell surface signal transduction and tumour promotion.蛋白激酶C在细胞表面信号转导及肿瘤促进中的作用。
Nature. 1984;308(5961):693-8. doi: 10.1038/308693a0.
8
[3H]ethylpropylamiloride, a radio-labelled diuretic for the analysis of the Na+/H+ exchange system. Its use with kidney cell membranes.[3H]乙基丙基氨氯吡脒，一种用于分析Na+/H+交换系统的放射性标记利尿剂。它在肾细胞膜中的应用。
EMBO J. 1984 Nov;3(11):2647-51. doi: 10.1002/j.1460-2075.1984.tb02188.x.
9
Thrombin stimulates Na+-H+ exchange across the human platelet plasma membrane.凝血酶刺激钠-氢交换穿过人血小板质膜。
FEBS Lett. 1984 Jul 9;172(2):272-4. doi: 10.1016/0014-5793(84)81139-4.
10
A role for inositol triphosphate in intracellular Ca2+ mobilization and granule secretion in platelets.肌醇三磷酸在血小板细胞内钙离子动员及颗粒分泌中的作用。
J Biol Chem. 1985 Dec 5;260(28):15172-9.
Zotero 插件