一些缺失氨基酸序列1 - 10的促肾上腺皮质激素片段对纯化的离体大鼠肾上腺细胞中皮质类固醇生成的异常作用。

The anomalous effect of some ACTH-fragments missing the amino acid sequence 1-10 on the corticosteroidogenesis in purified isolated rat adrenal cells.

作者信息

Goverde H J, Smals A G

出版信息

FEBS Lett. 1984 Jul 23;173(1):23-6. doi: 10.1016/0014-5793(84)81009-1.

Abstract

The corticosteroidogenicity of ACTH-derived peptides was tested in a purified isolated rat adrenal cell system. The activity was related to the activity of standard ACTH (synthetic hACTH; potency fixed at 100). The peptides ACTH NH2 and ACTH showed steroidogenicity at pharmacological doses (potencies: 0.00067 and 0.00032, respectively). None of the peptides tested inhibited or potentiated the ACTH-induced steroidogenesis at low doses (0.5-50 000 pg). The results suggest the presence of a second center within the ACTH molecule capable of inducing steroidogenesis.

摘要

在纯化的离体大鼠肾上腺细胞系统中测试了促肾上腺皮质激素衍生肽的皮质类固醇生成能力。该活性与标准促肾上腺皮质激素（合成人促肾上腺皮质激素；效价固定为100）的活性相关。促肾上腺皮质激素NH2和促肾上腺皮质激素肽在药理剂量下显示出类固醇生成能力（效价分别为0.00067和0.00032）。在低剂量（0.5 - 50000皮克）下，所测试的肽均未抑制或增强促肾上腺皮质激素诱导的类固醇生成。结果表明促肾上腺皮质激素分子内存在第二个能够诱导类固醇生成的中心。