Song E, Dusheiko G M, Bowyer S, Kew M C
Hepatology. 1984 Jul-Aug;4(4):608-10. doi: 10.1002/hep.1840040405.
Sera from 106 southern African blacks with hepatocellular carcinoma and hepatitis B surface antigenemia (HBsAg) were tested for hepatitis B viral DNA (HBV-DNA) activity, HBV-DNA polymerase concentrations, and HBV e antigen (HBeAg) and antibody (anti-HBe) to investigate the state of viral replication in these patients. HBeAg and anti-HBe were detected by radioimmunoassay, HBV-DNA by molecular hybridization using a 32p-labeled HBV-DNA probe, and HBV-DNA polymerase was measured by incorporation of 3H-labeled thymidine triphosphate into double-stranded HBV-DNA. HBeAg was present in 30.2% (32/106) of the patients, almost always in low titer; 63.8% of the patients were anti-HBe positive. Circulating HBV-DNA was detected in 18.8% (20/106) of patients, including 14 of 32 (43.7%) who were HBeAg positive and 6 of 74 (8.1%) who were anti-HBe positive. In most patients, only trace amounts of HBV-DNA were evident. Raised HBV-DNA polymerase activity was found in 5.6% (6/106) of the patients, all of whom were HBeAg positive and 4 of whom had detectable amounts of circulating HBV-DNA. The HBV-DNA polymerase activity was relatively low in these patients. HBV replication thus appears to be present in only a minority of southern African Blacks with HBV-related hepatocellular carcinoma, and when present is of low grade activity.
对106名患有肝细胞癌且乙肝表面抗原血症（HBsAg）呈阳性的南非黑人患者的血清进行检测，以分析乙肝病毒DNA（HBV-DNA）活性、HBV-DNA聚合酶浓度以及乙肝e抗原（HBeAg）和抗体（抗-HBe），从而研究这些患者的病毒复制状态。通过放射免疫分析法检测HBeAg和抗-HBe，使用32P标记的HBV-DNA探针通过分子杂交法检测HBV-DNA，并通过将3H标记的三磷酸胸腺嘧啶核苷掺入双链HBV-DNA中来测量HBV-DNA聚合酶。30.2%（32/106）的患者存在HBeAg，且几乎总是低滴度；63.8%的患者抗-HBe呈阳性。18.8%（20/106）的患者检测到循环HBV-DNA，其中包括32名HBeAg阳性患者中的14名（43.7%）和74名抗-HBe阳性患者中的6名（8.1%）。在大多数患者中，仅能检测到微量的HBV-DNA。5.6%（6/106）的患者发现HBV-DNA聚合酶活性升高，所有这些患者HBeAg均为阳性，其中4名患者可检测到循环HBV-DNA。这些患者的HBV-DNA聚合酶活性相对较低。因此，在与HBV相关的肝细胞癌的南非黑人患者中，似乎只有少数存在HBV复制，且即便存在，其活性也较低。
