Guan R, Yap I, Wong L, Tan L H, Oon C J, Wee A
Department of Medicine & Pathology, National University Hospital, Singapore.
Ann Acad Med Singap. 1989 Jan;18(1):8-11.
Sera from 60 consecutive patients with hepatocellular carcinoma (HCC) diagnosed by either a positive histology or at least two of the following: CT scan, hepatic ultrasound, hepatic angiogram and raised alpha-foetoprotein levels, were studied to determine their Hepatitis B surface antigen (HBsAg) status and the frequency of continuing viral replication. Markers of Hepatitis B virus (HBV) infection were detected using enzyme and radioimmunoassays. Hepatitis B DNA (HBV-DNA) was measured by molecular hybridization using a labelled recombinant HBV-DNA probe. Sera from 30 HBsAg positive 'healthy' carriers were used as controls. Forty-seven (78%) patients were HBsAg positive. Evidence of previous HBV infection was present in 12 of 13 HBsAg negative patients. 30% of HBsAg positive patients were HBeAg positive, 62% were anti-HBe positive and 8% had no 'e' markers. Serum HBV-DNA was present in 21 (45%) HBsAg positive patients: 10 (71%) HBeAg positive patients, 10 (35%) anti-HBe positive patients and 1 (25%) patient without any 'e' markers. Serum HBV-DNA levels were less than 0.6 ng/ml in 16 (76%) patients. HBV-DNA was absent in all HCC patients who were HBsAg negative. The frequency of serum HBV-DNA positivity was higher in 30 patients with HCC when compared with 30 age and sex matched healthy HBsAg positive individuals (57% vs 13% p less than 0.01). A sizeable proportion of HBsAg positive HCC patients have on-going viral replication and this is present in patients who were thought to have stopped replicating (anti-HBe positive patients).(ABSTRACT TRUNCATED AT 250 WORDS)
对60例经组织学检查确诊为肝细胞癌（HCC），或至少具备以下两项：CT扫描、肝脏超声、肝血管造影及甲胎蛋白水平升高的连续患者的血清进行研究，以确定其乙肝表面抗原（HBsAg）状态及持续病毒复制的频率。采用酶免疫法和放射免疫法检测乙肝病毒（HBV）感染标志物。使用标记的重组HBV-DNA探针通过分子杂交法检测乙肝DNA（HBV-DNA）。选取30例HBsAg阳性的“健康”携带者的血清作为对照。47例（78%）患者HBsAg阳性。13例HBsAg阴性患者中有12例有既往HBV感染证据。30%的HBsAg阳性患者HBeAg阳性，62%抗-HBe阳性，8%无“e”抗原标志物。21例（45%）HBsAg阳性患者血清中有HBV-DNA：10例（71%）HBeAg阳性患者、10例（35%）抗-HBe阳性患者及1例（25%）无任何“e”抗原标志物的患者。16例（76%）患者血清HBV-DNA水平低于0.6 ng/ml。所有HBsAg阴性的HCC患者血清中均未检测到HBV-DNA。与30例年龄和性别匹配的健康HBsAg阳性个体相比，30例HCC患者血清HBV-DNA阳性率更高（57%对13%，P<0.01）。相当一部分HBsAg阳性的HCC患者存在病毒持续复制，且在被认为已停止复制的患者（抗-HBe阳性患者）中也存在。（摘要截选于250词）
