转座子介导的霍乱弧菌诱变与重组
Transposon-mediated mutagenesis and recombination in Vibrio cholerae.
作者信息
Newland J W, Green B A, Holmes R K
出版信息
Infect Immun. 1984 Aug;45(2):428-32. doi: 10.1128/iai.45.2.428-432.1984.
Abstract
An efficient method for introducing transposons into the Vibrio cholerae chromosome or the P plasmid was developed by using F'tslac+ plasmids from Escherichia coli as suicide delivery vehicles. Hybrid P::Tn1, Tn5 and P::Tn1, Tn10 plasmids containing Tn5 or Tn10 in either of the two possible orientations were constructed. During conjugation, these hybrid plasmids mediated oriented transfer of markers from the sites of insertion of homologous transposons in the donor chromosome. The collection of donor strains described here permits oriented mobilization of all parts of the V. cholerae chromosome.
摘要
通过使用来自大肠杆菌的F'tslac + 质粒作为自杀性传递载体，开发出了一种将转座子导入霍乱弧菌染色体或P质粒的有效方法。构建了在两种可能方向上含有Tn5或Tn10的杂交P::Tn1、Tn5和P::Tn1、Tn10质粒。在接合过程中，这些杂交质粒介导了来自供体染色体中同源转座子插入位点的标记物的定向转移。这里描述的供体菌株集合允许霍乱弧菌染色体所有部分的定向移动。
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