Sublett R D, Romig W R
Infect Immun. 1981 Jun;32(3):1132-8. doi: 10.1128/iai.32.3.1132-1138.1981.
Transposon-facilitated recombination (Tfr) donors of classical Vibrio cholerae strain 162 were constructed by introducing the ampicillin transposon Tn1 into the P conjugative plasmid and the bacterial chromosome. The improved donors mediated high-frequency, polarized transfer of chromosomal genes from origins to confirm the gene orders of the previous classical strain 162 genetic map and to establish its circularity. Significant transfer of linked genes from E1 Tor Tfr donors to classical recipients was demonstrated, and other evidence for genetic relatedness of these two V. cholerae biotypes is discussed.
通过将氨苄青霉素转座子Tn1引入P型接合质粒和细菌染色体，构建了经典霍乱弧菌菌株162的转座子促进重组（Tfr）供体。改良后的供体介导了染色体基因从起始位点的高频、极性转移，从而确认了先前经典菌株162遗传图谱的基因顺序并确定了其环状结构。证明了E1 Tor Tfr供体的连锁基因向经典受体的显著转移，并讨论了这两种霍乱弧菌生物型遗传相关性的其他证据。
