兔骨骼肌和肺中钙激活中性蛋白酶缺乏组织特异性。

Lack of tissue-specificity of calcium-activated neutral proteases from skeletal muscle and lung of rabbit.

作者信息

Kawashima S, Ohsumi M, Hayashi M, Inomata M, Nakamura M, Imahori K

出版信息

J Biochem. 1984 Apr;95(4):1231-4. doi: 10.1093/oxfordjournals.jbchem.a134716.

DOI:10.1093/oxfordjournals.jbchem.a134716

Abstract

Calcium-activated neutral proteases (CANPs) with high sensitivity (microCANP) and low sensitivity (mCANP) to calcium ions were purified individually from rabbit skeletal muscle and rabbit lung and compared as to their electrophoretic properties, calcium requirements and peptide mapping of fragments produced by S. aureus V8 protease digestion of separated subunits. All of the results suggested that there is no difference between the microCANPs as well as between the mCANPs obtained from the two tissues, with respect to the chemical and enzymatic properties. However, the contents of CANPs in these tissues were different.

摘要

从兔骨骼肌和兔肺中分别纯化出对钙离子具有高敏感性（微钙激活中性蛋白酶，microCANP）和低敏感性（大钙激活中性蛋白酶，mCANP）的钙激活中性蛋白酶，并对它们的电泳性质、钙需求以及经金黄色葡萄球菌V8蛋白酶消化分离亚基产生的片段的肽图谱进行了比较。所有结果表明，就化学和酶学性质而言，从这两种组织中获得的微钙激活中性蛋白酶之间以及大钙激活中性蛋白酶之间没有差异。然而，这些组织中钙激活中性蛋白酶的含量是不同的。