Fujita N, Miwa T, Ishijima S, Izui K, Katsuki H
J Biochem. 1984 Apr;95(4):909-16. doi: 10.1093/oxfordjournals.jbchem.a134718.
The nucleotide sequence of the ppc gene, the structural gene for phosphoenolpyruvate carboxylase [EC 4.1.1.31], of Escherichia coli K-12 was determined. The gene codes for a polypeptide comprising 883 amino acid residues with a calculated molecular weight of 99,061. The amino acid sequence deduced from the nucleotide sequence was entirely consistent with the protein chemical data obtained with the purified enzyme, including the NH2- and COOH-terminal sequences and amino acid composition. The coding region is preceded by two putative ribosome binding sites, and is followed closely by a good representative of rho-independent terminator. The codon usage in the ppc gene suggests a moderate expression of the gene. The secondary structure of the enzyme was predicted from the deduced amino acid sequence.
测定了大肠杆菌K-12中磷酸烯醇丙酮酸羧化酶[EC 4.1.1.31]的结构基因ppc基因的核苷酸序列。该基因编码一个由883个氨基酸残基组成的多肽，计算分子量为99,061。从核苷酸序列推导的氨基酸序列与用纯化酶获得的蛋白质化学数据完全一致，包括NH2-和COOH-末端序列以及氨基酸组成。编码区之前有两个假定的核糖体结合位点，紧接着是一个很好的不依赖ρ的终止子代表。ppc基因中的密码子使用情况表明该基因表达适度。根据推导的氨基酸序列预测了该酶的二级结构。
