在PK(15)细胞中进行的微中和试验，用于检测抗跳跃病病毒抗体。
Microneutralization test in PK(15) cells for assay of antibodies to louping ill virus.
作者信息
Timoney P J, Geraghty V P, Harrington A M, Dillon P B
出版信息
J Clin Microbiol. 1984 Jul;20(1):128-30. doi: 10.1128/jcm.20.1.128-130.1984.
Abstract
A microneutralization test in PK(15) cells was developed to measure the neutralizing antibody response of a group of ponies experimentally challenged with louping ill virus. Viral cytopathic effect was maximal after 6 days of incubation, at which point titration endpoints were clear-cut and readily determinable. The assay compared favorably with the mouse neutralization test for accuracy and ease of performance.
摘要
在PK(15)细胞中开展了一项微量中和试验，以检测一组经跳跃病病毒实验性攻毒的小马的中和抗体反应。孵育6天后病毒细胞病变效应达到最大值，此时滴定终点明确且易于确定。该检测方法在准确性和操作简便性方面与小鼠中和试验相比具有优势。
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