Osamura R Y, Tsutsumi Y, Watanabe K
J Histochem Cytochem. 1984 Aug;32(8):885-93. doi: 10.1177/32.8.6086743.
Oncofetal aspects of ACTH and pro-opiomelanocortin (POMC)-derived peptides were studied immunohistochemically at the light and electron microscopic level in human fetal pituitary glands, pituitary adenomas, and small-cell carcinoma of the lung. ACTH, beta-endorphin, and gamma-MSH were localized in the same cells of both fetal and adult pituitary, as well as in the above-mentioned neoplastic tissues. However, alpha-MSH was observed only in the early fetal pituitary, its concentration decreasing with advancing gestational age. The adult pituitary contained only a few alpha-MSH-positive cells. By immunoelectron microscopy, ACTH in the adult pituitary was localized exclusively in the secretory granules. In fetal pituitary at 9 weeks' gestation, ACTH was localized in the perinuclear spaces (PNS), cisternae of rough endoplasmic reticulum (RER), Golgi saccules, and secretory granules. The staining pattern of ACTH in these organelles varied from cell to cell. In fetal pituitaries of greater gestational ages, ACTH was localized in secretory granules. The pituitary adenomas mimicked the staining characteristics of the adult pituitary, i.e., negative or only very occasional alpha-MSH staining and localization of ACTH in the secretory granules. The ectopic ACTH-producing tumors showed a staining pattern similar to that of the early fetal pituitary, i.e., positive staining for alpha-MSH and the presence of ACTH in PNS and cisternae of RER.
在光镜和电镜水平上，采用免疫组织化学方法研究了促肾上腺皮质激素（ACTH）和源自阿片促黑激素皮质素原（POMC）的肽类的肿瘤胎儿方面，研究对象包括人胎儿垂体、垂体腺瘤和肺小细胞癌。ACTH、β-内啡肽和γ-促黑素在胎儿和成人垂体的相同细胞中均有定位，在上述肿瘤组织中也有定位。然而，仅在胎儿早期垂体中观察到α-促黑素，其浓度随胎龄增加而降低。成人垂体仅含有少数α-促黑素阳性细胞。通过免疫电子显微镜观察，成人垂体中的ACTH仅定位于分泌颗粒中。在妊娠9周的胎儿垂体中，ACTH定位于核周间隙（PNS）、粗面内质网（RER）池、高尔基囊泡和分泌颗粒中。这些细胞器中ACTH的染色模式因细胞而异。在胎龄较大的胎儿垂体中，ACTH定位于分泌颗粒中。垂体腺瘤模仿成人垂体的染色特征，即α-促黑素染色阴性或仅偶尔呈阳性，且ACTH定位于分泌颗粒中。异位产生ACTH的肿瘤显示出与胎儿早期垂体相似的染色模式，即α-促黑素染色阳性，且PNS和RER池中存在ACTH。
