Drew H R
J Mol Biol. 1984 Jul 15;176(4):535-57. doi: 10.1016/0022-2836(84)90176-1.
Five commonly used nucleases were surveyed for their ability to distinguish among several different DNA backbone configurations. The digestion data suggest that: (1) DNAase I binds across the minor groove; whereas (2) nuclease S1 and (3) micrococcal nuclease bind to an exposed single strand; (4) copper/phenanthroline seeks a base-pair step; and (5) DNAase II requires just a stacked single strand of limited exposure. Only micrococcal nuclease is demonstrably base-specific, with a strong preference for T, A over C, G in any structural context.
研究了五种常用核酸酶区分几种不同DNA主链结构的能力。消化数据表明：（1）DNA酶I跨小沟结合；而（2）核酸酶S1和（3）微球菌核酸酶结合到暴露的单链上；（4）铜/菲咯啉寻找碱基对步；（5）DNA酶II只需要有限暴露的堆叠单链。只有微球菌核酸酶明显具有碱基特异性，在任何结构背景下都强烈偏好T、A而非C、G。
