Jarrett W F, Campo M S, Blaxter M L, O'Neil B W, Laird H M, Moar M H, Sartirana M L
J Natl Cancer Inst. 1984 Aug;73(2):499-504. doi: 10.1093/jnci/73.2.499.
Fibropapillomatosis of the upper alimentary canal of cattle is described. The tumors, found in the esophagus, esophageal groove, and rumen, showed involvement of the subepithelial fibroblasts as well as of the squamous epithelial layer. Although the fibropapilloma cells harbored multiple episomal copies of the genome of bovine papillomavirus type 2 (BPV-2) easily detected by hybridization techniques, no mature virus could be isolated from these lesions or seen by electron microscopy, and no viral antigen could be detected by immunohistochemical methods. It would appear, therefore, that within the limitations of the techniques employed the alimentary canal epithelium and the underlying fibroblasts, while allowing BPV-2 DNA replication, are nonpermissive for the expression of the viral vegetative functions and that transformation of the epithelial cells, like transformation of fibroblasts, can take place in the absence of infectious viral progeny.
描述了牛上消化道的纤维乳头瘤病。这些肿瘤见于食管、食管沟和瘤胃，显示上皮下成纤维细胞以及鳞状上皮层均受累。尽管通过杂交技术很容易检测到纤维乳头瘤细胞中含有牛乳头瘤病毒2型（BPV-2）基因组的多个游离型拷贝，但从这些病变中未能分离出成熟病毒，电镜下也未见病毒，免疫组化方法也未检测到病毒抗原。因此，在所用技术的局限性范围内，消化道上皮和其下方的成纤维细胞虽然允许BPV-2 DNA复制，但不允许病毒营养功能的表达，并且上皮细胞的转化，如同成纤维细胞的转化一样，可以在没有感染性病毒后代的情况下发生。
