Fujii K, Morio M, Kikuchi H, Ishihara S, Okida M, Ficor F
Life Sci. 1984 Jul 30;35(5):463-8. doi: 10.1016/0024-3205(84)90238-8.
Radical formation in vivo by anaerobic dehalogenation of halothane is described in this paper. The radicals were stabilized by spin-trapping and assayed by electron spin resonance spectrometry. The radical adducts were formed by inhalation of halothane in vivo and increased with decrease in inspired oxygen concentration. Following administration of the spin-trap, the expired concentration of CF2CHCl and CF3CH2Cl which are the anaerobic metabolites of halothane decreased, but bilious trifluoroacetate which are aerobic did not change. These results strongly suggest that radical intermediates are produced in anaerobic dehalogenation of halothane to CF2CHCl and CF3CH2Cl.
本文描述了氟烷在体内通过厌氧脱卤作用形成自由基的过程。自由基通过自旋捕获得以稳定，并通过电子自旋共振光谱法进行测定。自由基加合物在体内通过吸入氟烷形成，并随着吸入氧气浓度的降低而增加。给予自旋捕获剂后，氟烷的厌氧代谢产物CF2CHCl和CF3CH2Cl的呼出浓度降低，但需氧的胆汁三氟乙酸盐浓度未发生变化。这些结果有力地表明，在氟烷厌氧脱卤生成CF2CHCl和CF3CH2Cl的过程中会产生自由基中间体。
