Janzen E G, Towner R A, Krygsman P H, Haire D L, Poyer J L
Department of Chemistry and Biochemistry, University of Guelph, Ontario, Canada.
Free Radic Res Commun. 1990;9(3-6):343-51. doi: 10.3109/10715769009145693.
Free radicals were detected from the in vitro metabolism of halothane (rat liver microsomes) by the PBN spin trapping method. The detected radical species include the 1-chloro-2,2,2-trifluoro-1-ethyl radical (I), as determined by mass spectral analysis, and lipid-type radicals assigned by high resolution ESR spectroscopy with the use of d14-deuterated PBN. The lipid-derived radicals are a carbon-centred radical with the partially assigned structure CH2R and an oxygen-centred radical of the OR' type. From the mass spectral analysis of the spin adduct mixture there is also evidence for a halocarbon double adduct of PBN of the type I-PBN-I.
采用PBN自旋捕集法从氟烷（大鼠肝微粒体）的体外代谢中检测到了自由基。通过质谱分析确定，检测到的自由基种类包括1-氯-2,2,2-三氟-1-乙基自由基（I），以及使用d14-氘代PBN通过高分辨率电子顺磁共振光谱法确定的脂质型自由基。脂质衍生的自由基是具有部分确定结构CH2R的碳中心自由基和OR'型氧中心自由基。从自旋加合物混合物的质谱分析中也有证据表明存在I-PBN-I型的PBN卤代烃双加合物。
