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在大肠杆菌W39中对与噬菌体P1异免疫的广义转导噬菌体φw39的特性分析。

Characterization of generalized transducing phage phi w39 heteroimmune to phage P1 in Escherichia coli W39.

作者信息

Yoshida Y, Mise K

出版信息

Microbiol Immunol. 1984;28(4):415-26. doi: 10.1111/j.1348-0421.1984.tb00693.x.

DOI:10.1111/j.1348-0421.1984.tb00693.x
PMID:6087089
Abstract

Generalized transducing phage similar to phage P1 in Escherichia coli was isolated from E. coli W39, an antigenic test strain of the O121 group. This phage, designated phi w39, was reciprocally heteroimmune to phages P1 and P7, but nonreciprocally heteroimmune to phage D6. Transduction experiments using various R plasmids with different molecular weights suggested that phage phi w39 could transduce at least 65 megadaltons DNA. As in the case of P1 prophage, phi w39 prophage existed as a plasmid belonging to incompatibility group Y and carried a dnaB-like function. The molecular weight of phi w39 plasmid was nearly the same as that of plasmid, i.e., 58.6 megadaltons. Despite the pronounced structural and functional similarity of phages phi w39 and P1, restriction cleavage patterns of their genomes differed considerably.

摘要

从大肠杆菌O121群的抗原测试菌株W39中分离出了类似于大肠杆菌中P1噬菌体的广义转导噬菌体。这种噬菌体被命名为φw39,与P1和P7噬菌体相互异免疫,但与D6噬菌体非相互异免疫。使用各种不同分子量的R质粒进行的转导实验表明,噬菌体φw39可以转导至少65兆道尔顿的DNA。与P1原噬菌体的情况一样,φw39原噬菌体作为属于不相容性Y组的质粒存在,并具有类似dnaB的功能。φw39质粒的分子量与该质粒的分子量几乎相同,即58.6兆道尔顿。尽管噬菌体φw39和P1在结构和功能上有明显的相似性,但它们基因组的限制性切割模式有很大差异。

相似文献

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Characterization of generalized transducing phage phi w39 heteroimmune to phage P1 in Escherichia coli W39.在大肠杆菌W39中对与噬菌体P1异免疫的广义转导噬菌体φw39的特性分析。
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