Auerbach J, Howard-Flanders P
Mol Gen Genet. 1979 Jan 11;168(3):341-4. doi: 10.1007/BF00271506.
Lambda transducing phages carrying the excision repair genes uvrA+ and uvrC+ were selected from a pool of lambda phages carrying EcoR1 fragments of E. coli DNA. These phages and also lambdauvrB+ (obtained from Gottesman) were used to make lysogens of excision-defective strains carrying uvrA-, uvrB- or uvrC-. Lambda uvrA+ was found to transduce strains carrying uvrA- but not those carrying uvrB- or uvrC-, to normal ultraviolet resistance. Similarly, lambdauvrB+ and lambdauvrC+ were found to complement only the corresponding uvr- allele. The lambda transducing phages were co-transduced with gal+ by P1 phage into lysogenic gal- recipients, and presumably were integrated at the normal prophage site.
从携带大肠杆菌DNA的EcoR1片段的λ噬菌体库中筛选出携带切除修复基因uvrA⁺和uvrC⁺的λ转导噬菌体。这些噬菌体以及λuvrB⁺（从戈特斯曼处获得）被用于制备携带uvrA⁻、uvrB⁻或uvrC⁻的切除缺陷菌株的溶原菌。发现λuvrA⁺能将携带uvrA⁻的菌株转导至正常的紫外线抗性，但不能转导携带uvrB⁻或uvrC⁻的菌株。同样，发现λuvrB⁺和λuvrC⁺仅能互补相应的uvr⁻等位基因。λ转导噬菌体被P1噬菌体与gal⁺共转导到溶原性gal⁻受体中，推测它们整合在正常的原噬菌体位点。
