Quero A M, Daireaux M D, Fontanges R, Beaudry M Y, Laine G
Pathol Biol (Paris). 1984 Jun;32(5 Pt 2):636-9.
The authors propose a method for determining the virucidal potency of antiseptics, in which separation of antiseptic from virus is achieved by filtration through a gel of Sephadex LH20. The antiseptic is trapped within the gel while the virus passes through. Thus, the cytotoxic effect of the antiseptic, which usually hinders virus titration, is eliminated. Two filtration devices were developed: one is a polypropylene syringe, and the other is made of stainless steel. Determination of the effect of four products on poliovirus type I shows that this method allows classification of their activities.
作者们提出了一种确定防腐剂杀病毒效力的方法，其中通过经Sephadex LH20凝胶过滤实现防腐剂与病毒的分离。防腐剂被困在凝胶内，而病毒通过。因此，消除了通常会阻碍病毒滴定的防腐剂的细胞毒性作用。开发了两种过滤装置：一种是聚丙烯注射器，另一种由不锈钢制成。对四种产品对I型脊髓灰质炎病毒作用的测定表明，该方法能够对它们的活性进行分类。
