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酿酒酵母中α因子导向的成熟外源蛋白的合成与分泌。

Alpha-factor-directed synthesis and secretion of mature foreign proteins in Saccharomyces cerevisiae.

作者信息

Brake A J, Merryweather J P, Coit D G, Heberlein U A, Masiarz F R, Mullenbach G T, Urdea M S, Valenzuela P, Barr P J

出版信息

Proc Natl Acad Sci U S A. 1984 Aug;81(15):4642-6. doi: 10.1073/pnas.81.15.4642.

DOI:10.1073/pnas.81.15.4642
PMID:6087338
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC391546/
Abstract

Saccharomyces cerevisiae cells were transformed with plasmids containing hybrid genes in which the sequence encoding mature human epidermal growth factor was joined to sequences encoding the leader region (preprosegment) of the precursor of the yeast mating pheromone alpha-factor. These cells accurately process the hybrid protein and efficiently secrete authentic biologically active human epidermal growth factor into the medium.

摘要

用含有杂交基因的质粒转化酿酒酵母细胞，其中编码成熟人表皮生长因子的序列与编码酵母交配信息素α因子前体的前导区（前原区段）的序列相连。这些细胞能准确加工杂交蛋白，并有效地将真正具有生物活性的人表皮生长因子分泌到培养基中。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2572/391546/a483aa0d5218/pnas00616-0036-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2572/391546/a483aa0d5218/pnas00616-0036-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2572/391546/a483aa0d5218/pnas00616-0036-a.jpg

相似文献

1
Alpha-factor-directed synthesis and secretion of mature foreign proteins in Saccharomyces cerevisiae.酿酒酵母中α因子导向的成熟外源蛋白的合成与分泌。
Proc Natl Acad Sci U S A. 1984 Aug;81(15):4642-6. doi: 10.1073/pnas.81.15.4642.
2
[Human epidermal growth factor gene is expressed in saccharomyces cerevisiae].人表皮生长因子基因在酿酒酵母中表达。
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 1989 Oct;11(5):331-7.
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Secretion of mature mouse interleukin-2 by Saccharomyces cerevisiae: use of a general secretion vector containing promoter and leader sequences of the mating pheromone alpha-factor.酿酒酵母分泌成熟小鼠白细胞介素-2：使用含有交配信息素α-因子启动子和前导序列的通用分泌载体。
Gene. 1985;37(1-3):155-61. doi: 10.1016/0378-1119(85)90268-9.
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Alpha-factor-directed synthesis of Bacillus stearothermophilus alpha-amylase in Saccharomyces cerevisiae.α-因子导向的嗜热脂肪芽孢杆菌α-淀粉酶在酿酒酵母中的合成。
Biochem Biophys Res Commun. 1988 Apr 15;152(1):76-82. doi: 10.1016/s0006-291x(88)80682-x.
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Secretion of human epidermal growth factor from Saccharomyces cerevisiae using synthetic leader sequences.利用合成前导序列从酿酒酵母中分泌人表皮生长因子
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Synthesis and secretion of an Erwinia chrysanthemi pectate lyase in Saccharomyces cerevisiae regulated by different combinations of bacterial and yeast promoter and signal sequences.由细菌和酵母启动子及信号序列的不同组合调控的菊欧文氏菌果胶酸裂解酶在酿酒酵母中的合成与分泌。
Gene. 1992 Nov 2;121(1):35-45. doi: 10.1016/0378-1119(92)90159-m.
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An MF alpha 1-SUC2 (alpha-factor-invertase) gene fusion for study of protein localization and gene expression in yeast.用于研究酵母中蛋白质定位和基因表达的MFα1-SUC2（α-因子-转化酶）基因融合体。
Proc Natl Acad Sci U S A. 1983 Dec;80(23):7080-4. doi: 10.1073/pnas.80.23.7080.
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Protein secretion from Saccharomyces cerevisiae directed by the prepro-alpha-factor leader region.由前原α-因子前导区引导的酿酒酵母蛋白质分泌。
J Biol Chem. 1986 May 5;261(13):5858-65.
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Analysis of alpha-factor secretion signals by fusing with acid phosphatase of yeast.通过与酵母酸性磷酸酶融合分析α-因子分泌信号。
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Secretion of foreign proteins from Saccharomyces cerevisiae directed by alpha-factor gene fusions.由α-因子基因融合引导的酿酒酵母中外源蛋白的分泌。
Proc Natl Acad Sci U S A. 1984 Sep;81(17):5330-4. doi: 10.1073/pnas.81.17.5330.

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本文引用的文献

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A gas-liquid solid phase peptide and protein sequenator.一种气-液-固相肽和蛋白质测序仪。
J Biol Chem. 1981 Aug 10;256(15):7990-7.
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Two differentially regulated mRNAs with different 5' ends encode secreted with intracellular forms of yeast invertase.两个具有不同5'端且差异调控的mRNA编码酵母转化酶的细胞内形式和分泌形式。
Cell. 1982 Jan;28(1):145-54. doi: 10.1016/0092-8674(82)90384-1.
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An 'internal' signal sequence directs secretion and processing or proinsulin in bacteria.一个“内部”信号序列指导细菌中胰岛素原的分泌和加工。
Microorganisms. 2024 Feb 7;12(2):346. doi: 10.3390/microorganisms12020346.
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Current achievements, strategies, obstacles, and overcoming the challenges of the protein engineering in Pichia pastoris expression system.毕赤酵母表达系统中蛋白质工程的当前成就、策略、障碍及挑战应对
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Recombinant Production of Food Allergens in Yeast Pichia pastoris.毕赤酵母中食物过敏原的重组生产。
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Optimizing expression of Nanobody® molecules in Pichia pastoris through co-expression of auxiliary proteins under methanol and methanol-free conditions.通过甲醇和无甲醇条件下辅助蛋白的共表达优化毕赤酵母中 Nanobody®分子的表达。
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Improving therapeutic protein secretion in the probiotic yeast Saccharomyces boulardii using a multifactorial engineering approach.采用多因素工程方法提高益生菌酵母布拉氏酵母中治疗性蛋白质的分泌。
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Maximizing protein production by keeping cells at optimal secretory stress levels using real-time control approaches.通过实时控制方法使细胞保持在最佳分泌压力水平，从而最大限度地提高蛋白质产量。
Nat Commun. 2023 May 25;14(1):3028. doi: 10.1038/s41467-023-38807-9.
9
Full-length versus truncated α-factor secretory signal sequences for expression of recombinant human insulin precursor in yeast Pichia pastoris: a comparison.用于在毕赤酵母中表达重组人胰岛素原的全长与截短型α-因子分泌信号序列：一项比较
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10
The α-mating factor secretion signals and endogenous signal peptides for recombinant protein secretion in Komagataella phaffii.用于毕赤酵母中重组蛋白分泌的α-交配因子分泌信号和内源性信号肽。
Biotechnol Biofuels Bioprod. 2022 Dec 16;15(1):140. doi: 10.1186/s13068-022-02243-6.
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Distinct repressible mRNAs for cytoplasmic and secreted yeast invertase are encoded by a single gene.细胞质和分泌型酵母转化酶的不同可阻遏mRNA由单个基因编码。
Cell. 1981 Aug;25(2):525-36. doi: 10.1016/0092-8674(81)90071-4.
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Order of events in the yeast secretory pathway.酵母分泌途径中的事件顺序。
Cell. 1981 Aug;25(2):461-9. doi: 10.1016/0092-8674(81)90064-7.
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Stepwise cleavage of the pro part of promelittin by dipeptidylpeptidase IV. Evidence for a new type of precursor--product conversion.二肽基肽酶IV对前蜂毒溶血肽原部分的逐步切割。一种新型前体-产物转化的证据。
Eur J Biochem. 1980 Oct;111(1):49-58. doi: 10.1111/j.1432-1033.1980.tb06073.x.
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Oligonucleotide-directed mutagenesis using M13-derived vectors: an efficient and general procedure for the production of point mutations in any fragment of DNA.使用M13衍生载体的寡核苷酸定向诱变：在任何DNA片段中产生点突变的高效通用方法。
Nucleic Acids Res. 1982 Oct 25;10(20):6487-500. doi: 10.1093/nar/10.20.6487.
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Structure of a yeast pheromone gene (MF alpha): a putative alpha-factor precursor contains four tandem copies of mature alpha-factor.酵母信息素基因（MFα）的结构：一种假定的α-因子前体包含四个成熟α-因子的串联拷贝。
Cell. 1982 Oct;30(3):933-43. doi: 10.1016/0092-8674(82)90298-7.
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Glycosylation and processing of prepro-alpha-factor through the yeast secretory pathway.前原α因子在酵母分泌途径中的糖基化与加工过程。
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Chemical synthesis of a gene for human epidermal growth factor urogastrone and its expression in yeast.人表皮生长因子尿抑胃素基因的化学合成及其在酵母中的表达。
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