Huang B R
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 1989 Oct;11(5):331-7.
Saccharomyces cerevisiae was transformed with plasmids containing the synthetic hEGF gene under control of a alpha-factor promoter. The expressed protein was efficiently secreted into the medium broth and was shown to be a biologically active hEGF.
用含有在α-因子启动子控制下的合成人表皮生长因子(hEGF)基因的质粒转化酿酒酵母。表达的蛋白质被有效地分泌到培养基中,并显示具有生物活性的人表皮生长因子。