• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利:CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法,用中文像聊天一样搜索,搜遍4000万医学文献。AI智能推荐,让科研检索更轻松。

立即免费搜索

文件翻译

保留排版,准确专业,支持PDF/Word/PPT等文件格式,支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述,25分钟生成高质量综述,智能提取关键信息,辅助科研写作。

立即免费体验

人表皮生长因子基因在酿酒酵母中表达。

[Human epidermal growth factor gene is expressed in saccharomyces cerevisiae].

作者信息

Huang B R

出版信息

Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 1989 Oct;11(5):331-7.

PMID:2534612
Abstract

Saccharomyces cerevisiae was transformed with plasmids containing the synthetic hEGF gene under control of a alpha-factor promoter. The expressed protein was efficiently secreted into the medium broth and was shown to be a biologically active hEGF.

摘要

用含有在α-因子启动子控制下的合成人表皮生长因子(hEGF)基因的质粒转化酿酒酵母。表达的蛋白质被有效地分泌到培养基中,并显示具有生物活性的人表皮生长因子。

相似文献

1
[Human epidermal growth factor gene is expressed in saccharomyces cerevisiae].人表皮生长因子基因在酿酒酵母中表达。
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 1989 Oct;11(5):331-7.
2
Alpha-factor-directed synthesis and secretion of mature foreign proteins in Saccharomyces cerevisiae.酿酒酵母中α因子导向的成熟外源蛋白的合成与分泌。
Proc Natl Acad Sci U S A. 1984 Aug;81(15):4642-6. doi: 10.1073/pnas.81.15.4642.
3
Expression of small synthetic genes coding for hEGF, human epidermal growth factor, and CPTI II, serine proteinase inhibitor from Cucurbitacea, cloned in a novel expression/secretion vector in Saccharomyces cerevisiae.
Acta Biochim Pol. 1996;43(1):255-64.
4
Chemical synthesis, cloning and expression of human epidermal growth factor gene in Saccharomyces cerevisiae.人表皮生长因子基因的化学合成、克隆及在酿酒酵母中的表达
Chin J Biotechnol. 1991;7(4):253-61.
5
Secretion of human epidermal growth factor from Saccharomyces cerevisiae using synthetic leader sequences.利用合成前导序列从酿酒酵母中分泌人表皮生长因子
Gene. 1991 Oct 15;106(2):267-71. doi: 10.1016/0378-1119(91)90209-t.
6
Secretory expression of a single-chain insulin precursor in yeast and its conversion into human insulin.单链胰岛素前体在酵母中的分泌表达及其转化为人胰岛素。
Sci China C Life Sci. 1996 Jun;39(3):225-33.
7
The secretion of glucagon by transformed yeast strains.
FEBS Lett. 1987 Feb 23;212(2):302-6. doi: 10.1016/0014-5793(87)81365-0.
8
Cloning and expression of the hEGF gene in Saccharomyces cerevisiae.
Acta Biochim Pol. 1993;40(1):4-7.
9
Efficient synthesis of secreted murine interleukin-2 by Saccharomyces cerevisiae: influence of 3'-untranslated regions and codon usage.
Gene. 1992 Feb 15;111(2):207-13. doi: 10.1016/0378-1119(92)90688-l.
10
[Preparation of Saccharomyces cerevisiae strains with regulated MFalpha1 promotor activity].[具有调控型MFalpha1启动子活性的酿酒酵母菌株的制备]
Mol Gen Mikrobiol Virusol. 1991 Dec(12):14-9.