Faggioni A, Ablashi D V, Dahlberg J, Armstrong G, Sundar S K
Proc Soc Exp Biol Med. 1984 Sep;176(4):407-13. doi: 10.3181/00379727-176-41890.
Pre- and posttreatment with N-methyl-N'-nitro-nitrosoguanidine (MNNG) of owl monkey kidney (OMK) cells infected with Herpesvirus saimiri (HVS) resulted in one to three logs higher yields of virus, depending upon the dose of MNNG. A higher percentage of cells also showed HVS early antigen (EA) and late antigen (LA) by immunofluorescence when OMK cells infected with HVS were fed with medium containing MNNG. The high yields of HVS were also observed by electron microscopy. MNNG did not induce HVS-EA in HVS nonproducer lymphoblastoid T cells, nor did it enhance TPA-induced EA to LA. The data suggest that MNNG could be useful in obtaining high yields of virus and/or antigen-producing cells for immunofluorescence or other biochemical experiments, especially from those strains of HVS which grow poorly in vitro. The interaction of MNNG and HVS could also be useful for in vitro transformation or in vivo enhancement of the malignant process.
用N-甲基-N'-硝基-亚硝基胍（MNNG）对感染猴疱疹病毒（HVS）的猫头鹰猴肾（OMK）细胞进行预处理和后处理，根据MNNG的剂量，病毒产量可提高一到三个对数。当用含有MNNG的培养基培养感染HVS的OMK细胞时，通过免疫荧光检测也有更高比例的细胞显示出HVS早期抗原（EA）和晚期抗原（LA）。通过电子显微镜也观察到了HVS的高产率。MNNG不会在HVS非生产性淋巴母细胞样T细胞中诱导HVS-EA，也不会增强佛波酯（TPA）诱导的EA向LA的转化。数据表明，MNNG可用于获得高产率的病毒和/或用于免疫荧光或其他生化实验的抗原产生细胞，特别是对于那些在体外生长不良的HVS毒株。MNNG与HVS的相互作用也可能有助于体外转化或体内恶性过程的增强。
