Giddens W E
Lab Anim Sci. 1976 Dec;26(6 Pt 2):1113-26.
The lymphocytes of five owl monkeys (Aotus trivirgatus) infected with Herpesvirus saimiri and three control monkeys were studied. Lymphocytes were separated on Ficoll-Hypaque gradients, incubated in suspension cultures, and prepared for electron microscopic and immunofluorescent study at 24, 48, and 72 hours after the beginning of culture. Buffy coats of whole blood obtained immediately after bleeding were also prepared for study. At the time of the study, four of the five infected monkeys had died with malignant lymphoma and lymphocytic leukemia. Herpesvirus saimiri virions were demonstrated by electron microscopy and H saimiri antigens by immunofluorescence in 1-20% of the lymphocytes from infected monkey in two of five cultures at 24 hours after culture, four of five at 48 hours after culture, and all of five at 72 hours after culture. There was good agreement between the electron microscopic and the immunofluorescent data. None of the control monkey cultures and none of the buffy coat preparations contained H saimiri virions or antigens. By electron microscopy, the great majority of the virus particles were nucleocapsids within the nuclei of lymphocytes. Enveloped virions were seldom observed. There was some evidence to suggest that the higher the percentage of lymphocyte containing H saimiri, the poorer the prognosis for the monkey. Herpesvirus saimiri was isolated from all five infected monkeys by co-cultivation of lymphocytes with Vero cells. Control lymphocyte co-cultivations were negative.
对5只感染了猴疱疹病毒的夜猴（Aotus trivirgatus）和3只对照猴的淋巴细胞进行了研究。淋巴细胞通过Ficoll-Hypaque梯度分离，在悬浮培养中孵育，并在培养开始后的24、48和72小时制备用于电子显微镜和免疫荧光研究。出血后立即采集的全血的血沉棕黄层也制备用于研究。在研究时，5只感染猴中有4只死于恶性淋巴瘤和淋巴细胞白血病。在培养24小时后，5份培养物中的2份、培养48小时后5份中的4份以及培养72小时后5份中的所有培养物中，通过电子显微镜在感染猴的1%-20%的淋巴细胞中检测到猴疱疹病毒颗粒，通过免疫荧光检测到猴疱疹病毒抗原。电子显微镜和免疫荧光数据之间有很好的一致性。对照猴的培养物和血沉棕黄层制备物中均未含有猴疱疹病毒颗粒或抗原。通过电子显微镜观察，绝大多数病毒颗粒是淋巴细胞核内的核衣壳。很少观察到有包膜的病毒颗粒。有一些证据表明，含有猴疱疹病毒的淋巴细胞百分比越高，猴子的预后越差。通过将淋巴细胞与Vero细胞共培养，从所有5只感染猴中分离出了猴疱疹病毒。对照淋巴细胞共培养为阴性。
