构建一种质粒，该质粒含有功能正常的大肠杆菌uvrA、B和C基因，其结构可能适合哺乳动物表达。

Construction of a plasmid containing functional Escherichia coli uvrA, B, and C genes in a configuration potentially suitable for mammalian expression.

作者信息

DeLuca J G, Doetsch P W, Haseltine W A

出版信息

Plasmid. 1984 May;11(3):253-9. doi: 10.1016/0147-619x(84)90032-5.

Abstract

A plasmid, pUVABC-2, was constructed that encodes functional uvrA, B, and C genes of Escherichia coli. This plasmid also contains the gpt and ampr genes for positive selection in either bacterial or mammalian systems. Each of the uvrA, B, C, and gpt genes is located between SV40 initiation and termination signals and retains the original bacterial promoters. This recombinant vector conferred a wild-type UV resistance phenotype to uvrA-, B-, and C- strains of E. coli. The results indicate that each of the uvr genes contained in pUVABC-2 function in E. coli. The plasmid is a potential biological probe for DNA repair in mammalian cells.

摘要

构建了一种质粒pUVABC - 2，它编码大肠杆菌功能性的uvrA、uvrB和uvrC基因。该质粒还包含gpt和ampr基因，用于在细菌或哺乳动物系统中进行阳性选择。uvrA、uvrB、uvrC和gpt基因各自位于SV40起始和终止信号之间，并保留了原始的细菌启动子。这种重组载体赋予大肠杆菌uvrA -、uvrB -和uvrC -菌株野生型紫外线抗性表型。结果表明，pUVABC - 2中包含的每个uvr基因在大肠杆菌中都有功能。该质粒是一种用于哺乳动物细胞DNA修复的潜在生物学探针。