噬菌体T4 denV结构基因在大肠杆菌中的表达。
Expression of the bacteriophage T4 denV structural gene in Escherichia coli.
作者信息
Recinos A, Augustine M L, Higgins K M, Lloyd R S
出版信息
J Bacteriol. 1986 Nov;168(2):1014-8. doi: 10.1128/jb.168.2.1014-1018.1986.
Abstract
The expression of the T4 denV gene, which previously had been cloned in plasmid constructs downstream of the bacteriophage lambda hybrid promoter-operator oLpR, was analyzed under a variety of growth parameters. Expression of the denV gene product, endonuclease V, was confirmed in DNA repair-deficient Escherichia coli (uvrA recA) by Western blot analyses and by enhancements of resistance to UV irradiation.
摘要
T4噬菌体denV基因先前已克隆到噬菌体λ杂交启动子-操纵子oLpR下游的质粒构建体中，研究人员在各种生长参数下对其表达情况进行了分析。通过蛋白质免疫印迹分析以及增强对紫外线照射的抗性，在DNA修复缺陷型大肠杆菌（uvrA recA）中证实了denV基因产物核酸内切酶V的表达。
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