带有细菌和真菌标记物、携带T4噬菌体denV基因并恢复大肠杆菌uvrA紫外线抗性的杂种质粒
[Hybrid plasmid with bacterial and fungal markers carrying the denV gene of T4 phage and restoring the UV-resistance of E. coli uvrA].
作者信息
Bekker M L, Kaboev O K, Akhmedov A T, Smolina V S, Stepanova I M
出版信息
Mol Gen Mikrobiol Virusol. 1985 Dec(12):36-8.
The hybrid plasmid pYBP2 with bacterial (ampR), yeast (LEU2) and bacteriophage T4 (denV) genes has been constructed. The plasmid transformed Escherichia coli CSR603 uvrA recA ampS leuA phr- to ampicillin resistance, leucine independence, UV-resistance similar to the one of uvrA+ recA strain. Cell-free extracts of transformed Escherichia coli cells contain low level of ultraviolet-endonuclease activity in contrast to nontransformed cells containing no enzyme.
已构建出带有细菌（氨苄青霉素抗性基因ampR）、酵母（亮氨酸合成基因LEU2）和噬菌体T4（抗紫外线基因denV）基因的杂种质粒pYBP2。该质粒将大肠杆菌CSR603 uvrA recA ampS leuA phr-转化为对氨苄青霉素有抗性、无需亮氨酸且具有与uvrA+ recA菌株相似的抗紫外线能力。与不含该酶的未转化细胞相比，转化后的大肠杆菌细胞的无细胞提取物中含有低水平的紫外线内切酶活性。
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