源自多形核白细胞的一种因子对培养的大鼠滑膜细胞中前列腺素合成的刺激作用。

Stimulation of prostaglandin synthesis in cultured rat synovial cells by a factor derived from polymorphonuclear leukocytes.

作者信息

Hashida R, Kobayashi S, Shirota H, Yoshimatsu K, Ohsawa S, Hori H, Hattori S, Nagai Y

出版信息

Prostaglandins. 1984 May;27(5):697-709. doi: 10.1016/0090-6980(84)90008-x.

Abstract

Stimulation of synovial cell prostaglandin production by a factor obtained from casein-induced peritoneal polymorphonuclear (PMN) cells has been investigated. Both the extract and short time cultured medium of rat peritoneal PMN cells stimulate prostaglandin (PG)E2 production as well as collagenase production in the culture of rat synovial cells. PGE2 production by the cells in the presence of the PMN factor is much faster (5 to 24 hr) than collagenase production (24 hr or later, Biomedical Res. 3, 506-516, 1982). This stimulating factor is confirmed to be derived from PMN cells, based on the purification of the cells from peritoneal exudate cells by the Ficoll-Urographin method. Elution profile of the factor on gel filtration has indicated that both PGE2 and collagenase productions by synovial cells are stimulated by the same effluent fractions corresponding to molecular weights of 15,000 - 20,000 daltons and 30,000 - 40,000 daltons. These results suggest that PMN cells are involved in PG production as well as collagenase production in the inflamed tissue by stimulating connective tissue cells such as synovial cells.

摘要

对酪蛋白诱导的腹膜多形核（PMN）细胞产生的一种因子刺激滑膜细胞前列腺素生成进行了研究。大鼠腹膜PMN细胞的提取物和短期培养基均能刺激大鼠滑膜细胞培养物中前列腺素（PG）E2的生成以及胶原酶的生成。在PMN因子存在的情况下，细胞产生PGE2的速度（5至24小时）比产生胶原酶的速度（24小时或更晚，《生物医学研究》3，506 - 516，1982）快得多。基于通过Ficoll - Urographin方法从腹膜渗出细胞中纯化细胞，证实该刺激因子源自PMN细胞。该因子在凝胶过滤上的洗脱图谱表明，滑膜细胞产生PGE2和胶原酶均受到对应分子量为15,000 - 20,000道尔顿和30,000 - 40,000道尔顿的相同流出级分的刺激。这些结果表明，PMN细胞通过刺激诸如滑膜细胞等结缔组织细胞，参与了炎症组织中PG的产生以及胶原酶的产生。