Krane S M, Dayer J M, Simon L S, Byrne M S
Coll Relat Res. 1985 Mar;5(2):99-117. doi: 10.1016/s0174-173x(85)80033-9.
Adherent rheumatoid synovial cells produce and release into supernatant culture medium latent collagenase and PGE2. The levels of collagenase and PGE2 can be increased by a soluble factor present in mononuclear cell-conditioned medium, partially purified by gel-filtration, which has homologies with interleukin 1, and is produced by monocyte/macrophages. The synovial cell cultures produce collagens (procollagens) and fibronectin as well. The factor(s) present in the mononuclear cell conditioned medium which increases medium levels of collagenase PGE2 also stimulates synthesis of total protein as well as types I and III procollagen by the synovial cells. This stimulation by the monocyte factor is augmented in the presence of indomethacin, which blocks endogenous PGE2 production. Medium levels of fibronectin parallel those of procollagen. The addition of exogenous PGE2 abolishes the effect of indomethacin on collagen and fibronectin synthesis. These observations of mononuclear cell-mediated increases in fibronectin synthesis may account for the high levels of fibronectin found by others in rheumatoid synovium and synovial fluids as the increases in collagen synthesis might also explain the fibrosis observed in some rheumatoid joints.
附着的类风湿性滑膜细胞产生潜在的胶原酶和前列腺素E2并释放到上清培养基中。胶原酶和前列腺素E2的水平可被单核细胞条件培养基中存在的一种可溶性因子升高，该因子经凝胶过滤部分纯化，与白细胞介素1有同源性，由单核细胞/巨噬细胞产生。滑膜细胞培养物也产生胶原蛋白（前胶原）和纤连蛋白。单核细胞条件培养基中存在的增加胶原酶和前列腺素E2培养基水平的因子也刺激滑膜细胞合成总蛋白以及I型和III型前胶原。单核细胞因子的这种刺激在吲哚美辛存在下增强，吲哚美辛可阻断内源性前列腺素E2的产生。纤连蛋白的培养基水平与前胶原平行。添加外源性前列腺素E2可消除吲哚美辛对胶原和纤连蛋白合成的影响。这些关于单核细胞介导的纤连蛋白合成增加的观察结果可能解释了其他人在类风湿性滑膜和滑液中发现的高水平纤连蛋白，因为胶原合成的增加也可能解释在一些类风湿性关节中观察到的纤维化。
