Hashimoto K, Murakami K, Hattori T, Ota Z
Acta Med Okayama. 1984 Jun;38(3):261-7. doi: 10.18926/AMO/30349.
Plasma immunoreactive CRF measured by radioimmunoassay decreased rapidly after intravenous injection of synthetic ovine corticotropin releasing factor (CRF) and showed a bi-exponential decay curve in five macaca fuscatas. Half lives of plasma immunoreactive CRF were 5.8 +/- 1.4 (Mean +/- SEM) min for the fast component and 38.3 +/- 2.4 min for the slow component. A bolus injection of 5 micrograms/kg CRF significantly increased the plasma cortisol level. CRF at 5 micrograms/kg induced a delayed response of ACTH and cortisol. Arginine vasopressin (AVP) at 0.5 micrograms/kg induced a slight increase in plasma ACTH and cortisol, but AVP at 0.1 micrograms/kg evoked no significant increase. When 0.5 micrograms/kg CRF and 0.1 micrograms/kg AVP were administered simultaneously, significant ACTH and cortisol responses occurred. The results indicate that CRF and AVP act synergistically to stimulate ACTH secretion in vivo.
通过放射免疫分析法测定，在五只猕猴静脉注射合成羊促肾上腺皮质激素释放因子（CRF）后，血浆免疫反应性CRF迅速下降，并呈现双指数衰减曲线。血浆免疫反应性CRF快速成分的半衰期为5.8±1.4（平均值±标准误）分钟，慢速成分为38.3±2.4分钟。静脉注射5微克/千克CRF可显著提高血浆皮质醇水平。5微克/千克的CRF诱导促肾上腺皮质激素（ACTH）和皮质醇出现延迟反应。0.5微克/千克的精氨酸加压素（AVP）可使血浆ACTH和皮质醇略有升高，但0.1微克/千克的AVP未引起显著升高。当同时给予0.5微克/千克CRF和0.1微克/千克AVP时，出现了显著的ACTH和皮质醇反应。结果表明，CRF和AVP在体内协同作用以刺激ACTH分泌。
