Rivier C, Vale W
Endocrinology. 1983 Sep;113(3):939-42. doi: 10.1210/endo-113-3-939.
A possible interaction between synthetic ovine corticotropin-releasing factor (CRF) and arginine vasopressin (AVP) was tested in anesthetized and in freely moving rats. In animals whose endogenous CRF release was blocked by chlorpromazine-morphine-nembutal, AVP elicited a significantly lower maximum ACTH response than did CRF, whereas the concomitant injection of both peptides resulted in a marked potentiation of CRF-induced ACTH secretion. In freely moving rats, AVP was more potent than CRF (on an equimolar basis) in elevating plasma ACTH levels. Since immunoneutralization of endogenous CRF by the administration of anti-CRF serum significantly reduced ACTH release due to injected AVP in these animals, we suggest that at least part of the AVP-induced ACTH secretion observed in nonanesthetized rats may be due to a potentiation of endogenous CRF by exogenously administered AVP. These data support previous reports of an in vitro synergism between CRF and vasopressin, and emphasize the complex role played by the interaction of these two peptides on ACTH release in vivo.
在麻醉和自由活动的大鼠中测试了合成羊促肾上腺皮质激素释放因子（CRF）和精氨酸加压素（AVP）之间可能的相互作用。在内源性CRF释放被氯丙嗪-吗啡-戊巴比妥阻断的动物中，AVP引起的促肾上腺皮质激素（ACTH）最大反应明显低于CRF，而同时注射这两种肽会导致CRF诱导的ACTH分泌显著增强。在自由活动的大鼠中，（在等摩尔基础上）AVP在升高血浆ACTH水平方面比CRF更有效。由于通过给予抗CRF血清对内源性CRF进行免疫中和显著降低了这些动物中注射AVP引起的ACTH释放，我们认为在未麻醉大鼠中观察到的至少部分AVP诱导的ACTH分泌可能是由于外源性给予的AVP对内源性CRF的增强作用。这些数据支持了先前关于CRF和加压素在体外协同作用的报道，并强调了这两种肽相互作用在体内ACTH释放中所起的复杂作用。
