Young T M, Young J D
Biochim Biophys Acta. 1984 Sep 5;775(3):441-5. doi: 10.1016/0005-2736(84)90202-5.
Fusion of phospholipid vesicles with planar bilayer membranes occurs provided there is an intermembrane contact, which can be mediated by phospholipid-binding proteins, even in the absence of calcium. The firm attachment phase is then followed by the osmotically-driven fusion. These results show that hydrophobic proteins (not necessarily Ca2+-binding proteins) may enhance fusion by promoting contact of membranes. Such proteins may operate synergistically with Ca2+ to reduce the threshold concentration of Ca2+ needed for fusion of biological membranes. Protein-mediated intermembrane contact resulting in fusion may play a crucial role in the regulation and catalysis of biological fusion events.
只要存在膜间接触，磷脂囊泡就能与平面双层膜发生融合，这种接触可由磷脂结合蛋白介导，即使在没有钙的情况下也是如此。随后是由渗透压驱动的融合阶段。这些结果表明，疏水蛋白（不一定是钙结合蛋白）可能通过促进膜的接触来增强融合。这类蛋白可能与钙离子协同作用，以降低生物膜融合所需的钙离子阈值浓度。蛋白质介导的导致融合的膜间接触可能在生物融合事件的调节和催化中起关键作用。
