Sealy R C, Puzyna W, Kalyanaraman B, Felix C C
Biochim Biophys Acta. 1984 Aug 21;800(3):269-76. doi: 10.1016/0304-4165(84)90405-7.
Free radicals produced during the autoxidation of 3,4-dihydroxyphenylalanine (DOPA) and other catechol(amine)s to melanins have been studied using electron spin resonance spectroscopy. Magnetic parameters for the radical intermediates have been determined, allowing the radicals to be unambiguously identified. Three types of radical are formed: the primary radical from one-electron oxidation of the parent catechol(amine); and two secondary radicals, one formed via OH- substitution, the other via cyclization. The formation of these radical species can be linked to molecular products formed during catecholamine oxidation and melanin formation.
利用电子自旋共振光谱研究了3,4-二羟基苯丙氨酸（多巴）和其他儿茶酚（胺）自氧化成黑色素过程中产生的自由基。已确定了自由基中间体的磁参数，从而能够明确识别这些自由基。形成了三种类型的自由基：母体儿茶酚（胺）单电子氧化产生的初级自由基；以及两种次级自由基，一种通过羟基取代形成，另一种通过环化形成。这些自由基种类的形成与儿茶酚胺氧化和黑色素形成过程中产生的分子产物有关。
