[钙离子和环磷酸腺苷依赖性蛋白激酶对猪心脏纯化的NAD -异柠檬酸脱氢酶的调节作用]
[Regulation of purified NAD-isocitrate dehydrogenase from the pig heart by calcium ions and cAMP-dependent protein kinase].
作者信息
Kulinskiĭ V I, Kolpakova T V
出版信息
Biull Eksp Biol Med. 1984 Aug;98(8):188-90.
PMID:6087956
Abstract
Different cAMP-dependent protein kinases do not phosphorylate homogeneous NAD-isocitrate dehydrogenase (ICDH) and do not change its activity. Ca2+ (3 X 10(-7) = 10(-3) M) activates the enzyme throughout the process of purification including homogeneous enzyme by decreasing the KM for isocitrate. The calmodulin inhibitor trifluoperazine does not change ICDH activation.
摘要
不同的环磷酸腺苷（cAMP）依赖性蛋白激酶不会使均一的烟酰胺腺嘌呤二核苷酸（NAD）-异柠檬酸脱氢酶（ICDH）磷酸化，也不会改变其活性。钙离子（3×10⁻⁷ = 10⁻³M）在整个纯化过程中（包括均一酶）通过降低异柠檬酸的米氏常数（KM）来激活该酶。钙调蛋白抑制剂三氟拉嗪不会改变ICDH的激活状态。
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