Velumian A A
Brain Res. 1984 Jun;316(2):229-39. doi: 10.1016/0165-3806(84)90307-9.
Motoneurons were recorded intracellularly in the isolated perfused spinal cord of 10 - 16-day chick embryos. Inhibitory postsynaptic potentials (IPSPs) were present in motoneurones of all ages studied and could be evoked by both ventral white column and dorsal root stimulation. IPSPs produced by orthodromic stimulation displayed many features of mature vertebrate motoneuronal IPSPs including the chloride dependence and sensitivity to currents passed through the cell membrane. Strychnine and chloride-free solution produced marked disinhibitory effects in the spinal cord indicating the presence of inhibitory synapses in interneuronal circuits of at least 11-day and older embryos. Possible sources of descending inhibitory influences on motoneurones and some functional aspects are discussed. The results support the hypothesis that the inhibition starts in the embryonic chick spinal cord rather early, before the 10th day of development.
在10至16日龄鸡胚的离体灌流脊髓中对运动神经元进行细胞内记录。在所研究的所有年龄段的运动神经元中均存在抑制性突触后电位（IPSPs），并且可通过腹侧白质柱和背根刺激诱发。由顺向刺激产生的IPSPs表现出成熟脊椎动物运动神经元IPSPs的许多特征，包括对氯离子的依赖性以及对通过细胞膜的电流的敏感性。士的宁和无氯溶液在脊髓中产生明显的去抑制作用，表明在至少11日龄及以上胚胎的中间神经元回路中存在抑制性突触。讨论了对运动神经元下行抑制影响的可能来源以及一些功能方面。结果支持这样的假设，即抑制在胚胎鸡脊髓中发育的第10天之前很早就开始了。
