Roth G A, Bornstein M B
Brain Res. 1984 Jul;317(1):105-11. doi: 10.1016/0165-3806(84)90145-7.
These studies were designed to determine whether chemical parameters can be used to quantify the demyelinative potency of anti-CNS sera or other demyelinating factors as revealed by cultured nerve tissue. The activity of 2',3'-cyclic nucleotide 3'-phosphohydrolase (CNPase) and the amount of some major myelin lipids were measured in cultured embryonic mouse spinal cord during different stages of demyelination. Cultures exposed to anti-white matter serum for various times (6-60 h) or various concentrations (2.5-25%) showed a decrease (up to -70%) in CNPase activity. This change paralleled the degree of demyelination as evaluated by light microscopic examination. Cultures exposed to normal rabbit serum under the same conditions did not show changes in the amount of myelin or in the activity of CNPase. Conversely, changes in the lipid content did not correlate with the morphologic evaluation of demyelination. Glycolipids were found to decrease in cultures exposed to anti-white matter as well as in those exposed to normal rabbit sera. The decrease in cerebrosides was higher with anti-white matter serum than with normal serum, but sulfatides were altered in similar amounts in both groups. In contrast, under the same conditions, the amount of total protein or other lipid such as total cholesterol is not altered. Therefore, biochemical analysis of some myelin components could constitute a more sensitive and reliable quantitative method for early evaluation of the time course of demyelination in cultured nerve tissues.
这些研究旨在确定化学参数是否可用于量化抗中枢神经系统血清或其他脱髓鞘因子的脱髓鞘效力，正如培养的神经组织所显示的那样。在脱髓鞘的不同阶段，对培养的胚胎小鼠脊髓中2',3'-环核苷酸3'-磷酸水解酶（CNPase）的活性和一些主要髓磷脂脂质的含量进行了测量。暴露于抗白质血清不同时间（6 - 60小时）或不同浓度（2.5 - 25%）的培养物显示，CNPase活性下降（高达 - 70%）。这种变化与通过光学显微镜检查评估的脱髓鞘程度平行。在相同条件下暴露于正常兔血清的培养物，髓磷脂含量或CNPase活性未显示变化。相反，脂质含量的变化与脱髓鞘的形态学评估不相关。发现暴露于抗白质血清的培养物以及暴露于正常兔血清的培养物中糖脂均减少。抗白质血清组中脑苷脂的减少高于正常血清组，但两组中硫脂的变化量相似。相比之下，在相同条件下，总蛋白或其他脂质如总胆固醇的量没有改变。因此，对一些髓磷脂成分的生化分析可能构成一种更敏感、可靠的定量方法，用于早期评估培养神经组织中脱髓鞘的时间进程。
