Roth G A, Yu R K, Bornstein M B
J Neurochem. 1983 Dec;41(6):1710-7. doi: 10.1111/j.1471-4159.1983.tb00884.x.
Several biochemical parameters were analyzed in cultured embryonic mouse spinal cord during various stages of normal myelinogenesis or demyelination. In cultures demyelinated by exposure to anti-whole CNS tissue serum plus complement, the activity of 2',3'-cyclic nucleotide 3'-phosphohydrolase (EC 3.1.4.37) was decreased 70%, whereas in cultures that did not show morphological changes with complement-inactivated anti-CNS serum or anti-myelin basic protein serum, the activity was 30% lower than in control cultures. The lipid composition of these cultures was quantitated by means of high-performance thin-layer chromatography densitometry technique. Cultures with normal nutrient medium alone or with the addition of 5% normal rabbit serum plus 10% guinea pig serum had 30% of the total lipid content of that present in newborn mouse spinal cord of the corresponding age. There were, however, relatively more lysophospholipids, cholesterol esters, triglycerides, and free fatty acids and less phosphatidylethanolamine and galactolipids in cultures as compared with normal spinal cord. Explants demyelinated by exposure to anti-CNS serum plus complement demonstrated principally a 70% decrease in the content of galactolipids with respect to normal cultures. When complement was inactivated, total lipids increased 42% (with increases of 40-70% in individual lipids). Inclusion of anti-myelin basic protein serum plus complement in the medium produced no significant changes in the lipid composition of the cultures.
在正常髓鞘形成或脱髓鞘的不同阶段，对培养的胚胎小鼠脊髓中的几种生化参数进行了分析。在暴露于抗全中枢神经系统组织血清加补体而脱髓鞘的培养物中，2',3'-环核苷酸3'-磷酸水解酶（EC 3.1.4.37）的活性降低了70%，而在使用补体灭活的抗中枢神经系统血清或抗髓鞘碱性蛋白血清处理后未出现形态学变化的培养物中，该活性比对照培养物低30%。这些培养物的脂质组成通过高效薄层色谱光密度测定技术进行定量。仅使用正常营养培养基或添加5%正常兔血清加10%豚鼠血清的培养物，其总脂质含量为相应年龄新生小鼠脊髓中脂质含量的30%。然而，与正常脊髓相比，培养物中的溶血磷脂、胆固醇酯、甘油三酯和游离脂肪酸相对较多，而磷脂酰乙醇胺和半乳糖脂相对较少。暴露于抗中枢神经系统血清加补体而脱髓鞘的外植体，其半乳糖脂含量相对于正常培养物主要降低了70%。当补体被灭活时，总脂质增加了42%（个别脂质增加了40 - 70%）。在培养基中加入抗髓鞘碱性蛋白血清加补体，培养物的脂质组成未发生显著变化。
