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大肠杆菌热休克调节基因的核苷酸序列表明其蛋白质产物可能是一种转录因子。

Nucleotide sequence of the heat shock regulatory gene of E. coli suggests its protein product may be a transcription factor.

作者信息

Landick R, Vaughn V, Lau E T, VanBogelen R A, Erickson J W, Neidhardt F C

出版信息

Cell. 1984 Aug;38(1):175-82. doi: 10.1016/0092-8674(84)90538-5.

DOI:10.1016/0092-8674(84)90538-5
PMID:6088062
Abstract

We have sequenced a cloned segment of E. coli chromosomal DNA that includes the heat shock regulatory gene htpR. This segment contains an 852 nucleotide open reading frame bounded by transcriptional and translational signals. Both in vivo and in vitro the cloned segment produces a single protein that migrates in gels with the cellular protein (F33.4) implicated as the htpR product. Properties of a cloned fragment of the coding sequence truncated at the promoter-distal end are consistent with this assignment. The htpR gene product appears homologous to the sigma factor of RNA polymerase, and the two proteins are predicted to have similar secondary structure. In addition, two regions of the predicted htpR product resemble protein-DNA contact points conserved in known DNA-binding proteins.

摘要

我们对大肠杆菌染色体DNA的一个克隆片段进行了测序，该片段包含热休克调节基因htpR。这个片段含有一个852个核苷酸的开放阅读框，其两侧为转录和翻译信号。在体内和体外，该克隆片段都产生一种单一蛋白质，它在凝胶中与被认为是htpR产物的细胞蛋白质（F33.4）一起迁移。在启动子远端截断的编码序列克隆片段的特性与此归属一致。htpR基因产物似乎与RNA聚合酶的σ因子同源，并且预测这两种蛋白质具有相似的二级结构。此外，预测的htpR产物的两个区域类似于已知DNA结合蛋白中保守的蛋白质-DNA接触点。

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1
Nucleotide sequence of the heat shock regulatory gene of E. coli suggests its protein product may be a transcription factor.大肠杆菌热休克调节基因的核苷酸序列表明其蛋白质产物可能是一种转录因子。
Cell. 1984 Aug;38(1):175-82. doi: 10.1016/0092-8674(84)90538-5.
2
Nucleotide sequence of katF of Escherichia coli suggests KatF protein is a novel sigma transcription factor.大肠杆菌katF的核苷酸序列表明KatF蛋白是一种新型的σ转录因子。
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The htpR gene product of E. coli is a sigma factor for heat-shock promoters.大肠杆菌的htpR基因产物是热休克启动子的一种σ因子。
Cell. 1984 Sep;38(2):383-90. doi: 10.1016/0092-8674(84)90493-8.
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Expression of ClpB, an analog of the ATP-dependent protease regulatory subunit in Escherichia coli, is controlled by a heat shock sigma factor (sigma 32).ClpB是大肠杆菌中一种依赖ATP的蛋白酶调节亚基的类似物，其表达受热休克σ因子（σ32）控制。
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Heat shock regulatory gene (htpR) of Escherichia coli is required for growth at high temperature but is dispensable at low temperature.大肠杆菌的热休克调节基因（htpR）是高温生长所必需的，但在低温下并非必需。
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Isolation and sequence analysis of rpoH genes encoding sigma 32 homologs from gram negative bacteria: conserved mRNA and protein segments for heat shock regulation.革兰氏阴性菌中编码σ32同源物的rpoH基因的分离与序列分析：热休克调节的保守mRNA和蛋白质区段
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Expression of ToxR, the transcriptional activator of the virulence factors in Vibrio cholerae, is modulated by the heat shock response.霍乱弧菌毒力因子的转录激活因子ToxR的表达受热休克反应调控。
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Sequence analysis and regulation of the htrA gene of Escherichia coli: a sigma 32-independent mechanism of heat-inducible transcription.大肠杆菌htrA基因的序列分析与调控：一种不依赖于σ32的热诱导转录机制
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Heat shock regulatory gene htpR influences rates of protein degradation and expression of the lon gene in Escherichia coli.热休克调节基因htpR影响大肠杆菌中蛋白质降解速率和lon基因的表达。
Proc Natl Acad Sci U S A. 1984 Nov;81(21):6647-51. doi: 10.1073/pnas.81.21.6647.

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