人血浆中免疫反应性γ-促黑素的测定。

Measurement of immunoreactive gamma-MSH in human plasma.

作者信息

Hale A C, Ratter S J, Tomlin S J, Lytras N, Besser G M, Rees L H

出版信息

Clin Endocrinol (Oxf). 1984 Aug;21(2):139-48. doi: 10.1111/j.1365-2265.1984.tb03453.x.

DOI:10.1111/j.1365-2265.1984.tb03453.x

Abstract

A radioimmunoassay for immunoreactive gamma-MSH (IR-gamma-MSH) in human plasma has been developed. The assay is capable of detecting normal basal circulating levels which range from less than 20-100 ng/1 at 0900 h. Plasma levels are raised concomitantly with ACTH during insulin induced hypoglycaemia and CRF stimulation and suppressed with dexamethasone. Chromatographic characterisation of IR-gamma-MSH in plasma demonstrates a major peak of IR-gamma-MSH, corresponding to purified glycosylated N-terminal pro-opiomelanocortin 1-76, when IR-gamma-MSH is secreted from the pituitary. In contrast IR-gamma-MSH produced ectopically appears to be heterogeneous.

摘要

已开发出一种用于检测人血浆中免疫反应性γ-促黑素（IR-γ-MSH）的放射免疫分析法。该分析法能够检测到正常基础循环水平，在上午9点时，其范围为低于20 - 100 ng/1。在胰岛素诱导的低血糖和促肾上腺皮质激素释放因子（CRF）刺激期间，血浆水平与促肾上腺皮质激素（ACTH）同时升高，而在地塞米松作用下则受到抑制。血浆中IR-γ-MSH的色谱特征表明，当IR-γ-MSH从垂体分泌时，有一个主要的IR-γ-MSH峰，对应于纯化的糖基化N端阿黑皮素原1 - 76。相比之下，异位产生的IR-γ-MSH似乎是异质性的。