Knecht E, Hernández-Yago J, Grisolía S
Exp Cell Res. 1984 Sep;154(1):224-32. doi: 10.1016/0014-4827(84)90682-7.
The role of the lysosomal system in accelerated protein degradation was investigated in 3T3 mouse fibroblasts and in the SV40 virus-transformed derivative, SV3T3. Rates of protein degradation and quantitative electron microscopic alterations in the lysosomal system were compared under four different growing conditions: exponential growth, confluent phase, serum deprivation, and confluent phase together with serum deprivation. We found a significant correlation between increases in rates of proteolysis of long-lived proteins and fractional volume of lysosomes, suggesting a causal relationship between the two, as well as a morphological explanation for the differences in rates of protein degradation in transformed and non-transformed cultured cells. The increase in lysosomal fractional volume resulted from an increase in dense bodies only (in serum-deprived exponential or confluent cultures) or from an increase in autophagic vacuoles and dense bodies (in serum-supplemented confluent cultures).
在3T3小鼠成纤维细胞和SV40病毒转化的衍生物SV3T3中，研究了溶酶体系统在加速蛋白质降解中的作用。在四种不同的生长条件下比较了蛋白质降解速率和溶酶体系统中的定量电子显微镜变化：指数生长期、汇合期、血清剥夺以及汇合期加血清剥夺。我们发现长寿命蛋白质的蛋白水解速率增加与溶酶体的分数体积之间存在显著相关性，这表明两者之间存在因果关系，同时也为转化和未转化的培养细胞中蛋白质降解速率的差异提供了形态学解释。溶酶体分数体积的增加仅源于致密体的增加（在血清剥夺的指数期或汇合期培养物中）或自噬泡和致密体的增加（在补充血清的汇合期培养物中）。
