Crespi C L, Thilly W G
Mutat Res. 1984 Sep;128(2):221-30. doi: 10.1016/0027-5107(84)90110-6.
A novel quantitative gene-locus mutation assay has been developed using a line of human lymphoblast cells, designated AHH-1, competent in oxidative xenobiotic metabolism. AHH-1 cells are sensitive to the mutagenic action of both chemically reactive mutagens and mutagens which require oxidative metabolism to exert their mutagenicity. These cells are readily mutated by direct exposure to ethyl methanesulfonate, ICR-191, 2-acetoaminofluorene, aflatoxin B1, benzo[a]pyrene (BP), cyclopenta[c, d]pyrene, dimethylnitrosamine, lasiocarpine, and 1-methylphenanthrene.
利用一株具有氧化外源性生物代谢能力的人类淋巴母细胞系（命名为AHH-1），开发了一种新型的定量基因座突变检测方法。AHH-1细胞对化学反应性诱变剂和需要氧化代谢来发挥其诱变性的诱变剂的诱变作用均敏感。通过直接暴露于甲磺酸乙酯、ICR-191、2-乙酰氨基芴、黄曲霉毒素B1、苯并[a]芘（BP）、环戊[c,d]芘、二甲基亚硝胺、毛果天芥菜碱和1-甲基菲，这些细胞很容易发生突变。
