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Identification of the replicative intermediates in SV40 DNA replication in vitro.

作者信息

Ariga H

出版信息

Nucleic Acids Res. 1984 Aug 10;12(15):6053-62. doi: 10.1093/nar/12.15.6053.

DOI:10.1093/nar/12.15.6053
PMID:6089107
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC320056/
Abstract

The soluble replication system is which the exogenously added simian virus 40 (SV40) DNA can be replicated semiconservatively in vitro, has been developed (Ariga and Sugano, J.Virol. 48, 481, 1983). This paper further characterized the in vitro products synthesized on the cloned DNA containing the origin of SV40 DNA replication. The time course and pluse-chase experiments showed that the in vitro products were converted from the open circle to closed circles having the various superhelical densities, and finally to the twisted formI DNA seen in vivo by the analysis of agarose gel electrophoresis, alkaline sucrose gradient centrifugation, and density-transfer in isopycnic centrifugation. The replicative intermediates isolated after the short term incubation had replicated strands of the size smaller than the full length, most of which correspond to that of the putative Okazaki fragment. These and the previous results indicate that this in vitro system should be useful to investigate the molecular mechanism of SV40 DNA replication.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c31a/320056/dc02b7ccd5bb/nar00333-0112-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c31a/320056/b617bc5da55e/nar00333-0109-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c31a/320056/caa43c845694/nar00333-0110-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c31a/320056/dc02b7ccd5bb/nar00333-0112-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c31a/320056/b617bc5da55e/nar00333-0109-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c31a/320056/caa43c845694/nar00333-0110-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c31a/320056/dc02b7ccd5bb/nar00333-0112-a.jpg

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引用本文的文献

1
Autonomous replicating sequences from mouse cells which can replicate in mouse cells in vivo and in vitro.来自小鼠细胞的自主复制序列，其能够在体内和体外的小鼠细胞中进行复制。
Mol Cell Biol. 1987 Jan;7(1):1-6. doi: 10.1128/mcb.7.1.1-6.1987.
2
Simian virus 40 DNA replication in vitro: purification and characterization of replication factors from mouse cells.
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3
Cloned mouse DNA fragments can replicate in a simian virus 40 T antigen-dependent system in vivo and in vitro.克隆的小鼠DNA片段能够在体内和体外的猿猴病毒40 T抗原依赖系统中进行复制。

本文引用的文献

1
Eukaryotic DNA replication: viral and plasmid model systems.真核生物DNA复制：病毒和质粒模型系统
Annu Rev Biochem. 1982;51:901-34. doi: 10.1146/annurev.bi.51.070182.004345.
2
Initiation of simian virus 40 DNA replication in vitro.体外猿猴病毒40 DNA复制的起始
J Virol. 1983 Nov;48(2):481-91. doi: 10.1128/JVI.48.2.481-491.1983.
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Binding of an analog of the simian virus 40 T antigen to wild-type and mutant viral replication origins.猿猴病毒40 T抗原类似物与野生型及突变型病毒复制起点的结合。
Mol Cell Biol. 1985 Mar;5(3):563-8. doi: 10.1128/mcb.5.3.563-568.1985.
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Alternative interactions of the SV40 A protein with DNA.猴空泡病毒40 A蛋白与DNA的交替相互作用。
Virology. 1981 Nov;115(1):75-87. doi: 10.1016/0042-6822(81)90090-8.
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Multiple rounds of adenovirus DNA synthesis in vitro.体外进行多轮腺病毒DNA合成。
Proc Natl Acad Sci U S A. 1981 Mar;78(3):1476-80. doi: 10.1073/pnas.78.3.1476.
6
Construction and analysis of simian virus 40 origins defective in tumor antigen binding and DNA replication.在肿瘤抗原结合和DNA复制方面存在缺陷的猿猴病毒40起源的构建与分析。
Proc Natl Acad Sci U S A. 1980 Nov;77(11):6491-5. doi: 10.1073/pnas.77.11.6491.
7
Origin-defective mutants of SV40.SV40的起源缺陷型突变体
Cold Spring Harb Symp Quant Biol. 1980;44 Pt 1,:293-300. doi: 10.1101/sqb.1980.044.01.033.
8
Cold-sensitive regulatory mutants of simian virus 40.猿猴病毒40的冷敏感调节突变体
J Mol Biol. 1980 Jun 15;140(1):129-42. doi: 10.1016/0022-2836(80)90359-9.
9
Bidirectional replication of Simian Virus 40 DNA.猿猴病毒40型DNA的双向复制
Proc Natl Acad Sci U S A. 1972 Nov;69(11):3097-100. doi: 10.1073/pnas.69.11.3097.
10
Simian virus 40 deoxyribonucleic acid synthesis: the viral replicon.猴病毒40脱氧核糖核酸合成：病毒复制子。
J Virol. 1972 Oct;10(4):591-8. doi: 10.1128/JVI.10.4.591-598.1972.