通过高压液相色谱法（HPLC）纯化单碘化血管活性肠肽（M125I-VIP）。
Purification of monoiodinated vasointestinal peptide (M125I-VIP) by high pressure liquid chromatography (HPLC).
作者信息
Marie J C, Boissard C, Rosselin G
出版信息
Peptides. 1984 Mar-Apr;5(2):179-82. doi: 10.1016/0196-9781(84)90203-1.
PMID:6089129
Abstract
In our developed reverse phase high performance liquid chromatography, four forms of M125I-VIP have been isolated free from unlabeled VIP and other iodinated components. The quicker eluting M125I-VIP forms (oxidised and reduced) have a consistently and significantly low non-specific binding with specific target cells of VIP (HT-29) as compared to the late eluting forms of VIP. The retention time is considerably increased when the molecule of VIP is fully iodinated.
摘要
在我们开发的反相高效液相色谱法中，已分离出四种形式的M125I - VIP，未含有未标记的VIP和其他碘化成分。与VIP的后期洗脱形式相比，洗脱较快的M125I - VIP形式（氧化型和还原型）与VIP的特定靶细胞（HT - 29）的非特异性结合始终显著较低。当VIP分子完全碘化时，保留时间会显著增加。
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