希拉毒蜥毒液与大鼠胰腺膜中促胰液素受体的相互作用。

Interaction of Gila monster venom with secretin receptors in rat pancreatic membranes.

作者信息

Gillet L, Robberecht P, Waelbroeck M, Camus J C, De Neef P, König W, Christophe J

出版信息

Peptides. 1984 Mar-Apr;5(2):407-9. doi: 10.1016/0196-9781(84)90244-4.

Abstract

The stimulatory effect of Gila monster venom on adenylate cyclase activity in rat pancreatic membranes was compared to that of porcine secretin and porcine VIP. The maximal effect exerted by the venom was identical to that of VIP but significantly lower than that of secretin. The effect of Gila monster venom could, however, be attributed to its interaction with secretin receptors rather than with VIP receptors, at variance with its previously described action on guinea pig pancreatic acini. Adenylate cyclase activation by both Gila monster venom and secretin in rat pancreatic membranes was, indeed: (1) dose-dependently inhibited by two secretin fragments secretin-(4-27) and secretin-(7-27), and (2) more severely depressed than VIP stimulation, after pretreating pancreatic membranes with dithiothreitol (DTT).

摘要

将吉拉毒蜥毒液对大鼠胰腺膜中腺苷酸环化酶活性的刺激作用与猪促胰液素和猪血管活性肠肽的刺激作用进行了比较。毒液产生的最大作用与血管活性肠肽的相同，但明显低于促胰液素的。然而，吉拉毒蜥毒液的作用可能归因于其与促胰液素受体的相互作用，而非与血管活性肠肽受体的相互作用，这与其先前描述的对豚鼠胰腺腺泡的作用不同。实际上，大鼠胰腺膜中吉拉毒蜥毒液和促胰液素对腺苷酸环化酶的激活作用：(1) 受到两种促胰液素片段促胰液素-(4-27) 和促胰液素-(7-27) 的剂量依赖性抑制；(2) 在用二硫苏糖醇 (DTT) 预处理胰腺膜后，比血管活性肠肽刺激更严重地受到抑制。