Grunditz T, Persson P, Håkanson R, Absood A, Böttcher G, Rerup C, Sundler F
Department of Otolaryngology, Malmö General Hospital, Sweden.
Proc Natl Acad Sci U S A. 1989 Feb;86(4):1357-61. doi: 10.1073/pnas.86.4.1357.
Helodermin is a vasoactive intestinal peptide-like peptide in the salivary gland venom of the lizard Heloderma suspectum. Helodermin-like immunofluorescence was observed in the parafollicular (C) cells in several mammals and in the C cell homologues of the chicken ultimobranchial gland. Thus, helodermin-like peptides coexist with calcitonin. The results of radioimmunoassay agreed with the immunocytochemical findings. HPLC of rat thyroid extracts revealed one major peak of helodermin-like immunoreactivity, which eluted in a position close to that of lizard helodermin. Helodermin stimulated basal thyroid hormone secretion and colloid droplet formation in conscious mice. The effect of large doses of helodermin was quite long-lasting and the maximal response occurred after 2-6 hr. In addition, helodermin suppressed the incorporation of calcium into bone in conscious rats. The findings suggest that helodermin-like peptides in C cells may be involved in the local regulation of thyroid hormone secretion and in the maintenance of calcium homeostasis.
蜥蜴毒液中的类血管活性肠肽——铃蟾肽，存在于毒蜥（Heloderma suspectum）唾液腺毒液中。在几种哺乳动物的滤泡旁（C）细胞以及鸡后鳃腺的C细胞同源物中观察到了类铃蟾肽免疫荧光。因此，类铃蟾肽与降钙素共存。放射免疫分析结果与免疫细胞化学发现一致。大鼠甲状腺提取物的高效液相色谱显示出一个主要的类铃蟾肽免疫反应峰，其洗脱位置与蜥蜴铃蟾肽相近。铃蟾肽刺激清醒小鼠的基础甲状腺激素分泌和胶体小滴形成。大剂量铃蟾肽的作用持续时间很长，最大反应在2 - 6小时后出现。此外，铃蟾肽抑制清醒大鼠骨骼中钙的掺入。这些发现表明，C细胞中的类铃蟾肽可能参与甲状腺激素分泌的局部调节以及钙稳态的维持。
