Bruns M, Lehmann-Grube F
Virology. 1984 Aug;137(1):49-57. doi: 10.1016/0042-6822(84)90007-2.
Large numbers of VSV (LCMV) pseudotypes with the genomes of vesicular stomatitis virus (VSV) and the coat proteins of lymphocytic choriomeningitis virus (LCMV) were produced by infecting L cells first with LCMV and subsequently with VSV, the latter in the presence of tunicamycin. Separation by gradient centrifugation from the concomitantly produced LCMV genotypes, followed by polyacrylamide gel electrophoresis (PAGE), failed to reveal measurable quantities of the one glycoprotein ("G") of VSV. By serologic analysis it could be shown that anti-VSV antibody still attached, although with low efficiency. VSV (LCMV) retained its infectivity during purification. Reversal of the sequence of infection under otherwise identical conditions led to the formation of LCMV (VSV) pseudotypes. When separated from VSV genotypes, PAGE did not disclose glycoproteins of LCMV, and serologic analysis failed to detect attachment of anti-LCM virus antibody. LCMV (VSV) lost its infectivity during purification.
通过先将淋巴细胞性脉络丛脑膜炎病毒（LCMV）感染L细胞，随后在衣霉素存在的情况下用水泡性口炎病毒（VSV）感染，产生了大量具有水泡性口炎病毒基因组和淋巴细胞性脉络丛脑膜炎病毒衣壳蛋白的VSV（LCMV）假型。通过梯度离心从同时产生的LCMV基因型中分离，随后进行聚丙烯酰胺凝胶电泳（PAGE），未能检测到可测量数量的VSV的一种糖蛋白（“G”）。通过血清学分析可以表明，抗VSV抗体仍然附着，尽管效率较低。VSV（LCMV）在纯化过程中保留了其感染性。在其他条件相同的情况下，颠倒感染顺序会导致形成LCMV（VSV）假型。当从VSV基因型中分离时，PAGE未显示LCMV的糖蛋白，血清学分析也未能检测到抗LCM病毒抗体的附着。LCMV（VSV）在纯化过程中失去了其感染性。
