对胰岛素刺激的环磷酸腺苷磷酸二酯酶降低肝细胞内循环磷酸腺苷浓度能力的评估。
An assessment of the ability of insulin-stimulated cyclic AMP phosphodiesterase to decrease hepatocyte intracellular cyclic AMP concentrations.
作者信息
Heyworth C M, Wallace A V, Wilson S R, Houslay M D
出版信息
Biochem J. 1984 Aug 15;222(1):183-7. doi: 10.1042/bj2220183.
Abstract
Treatment of hepatocytes with either NH4Cl (10mM) or fructose (10mM) blocks insulin's activation of the 'dense-vesicle' cyclic AMP phosphodiesterase. The ability of insulin (10 nM) to decrease intracellular cyclic AMP concentrations raised by glucagon (10 nM) was unaffected by pre-treatment with either NH4Cl (10 mM) or fructose (10 mM). It is concluded that the 'dense-vesicle' enzyme does not play a significant role in this action of insulin and that as yet unidentified cyclic AMP phosphodiesterase(s) must be activated by insulin. Treatment of hepatocytes with either NH4Cl or fructose appeared to increase, reversibly, cyclic AMP phosphodiesterase activity. When N6-(phenylisopropyl)adenosine was used to prevent glucagon from blocking insulin's activation of the plasma-membrane cyclic AMP phosphodiesterase activity, insulin's ability to decrease intracellular cyclic AMP concentrations in glucagon-treated hepatocytes was increased markedly. Insulin's activation of the plasma-membrane cyclic AMP phosphodiesterase activity can exert a potent effect in decreasing intracellular cyclic AMP concentrations elevated by glucagon.
摘要
用氯化铵（10 mM）或果糖（10 mM）处理肝细胞会阻断胰岛素对“致密囊泡”环磷酸腺苷磷酸二酯酶的激活作用。胰岛素（10 nM）降低由胰高血糖素（10 nM）升高的细胞内环磷酸腺苷浓度的能力不受氯化铵（10 mM）或果糖（10 mM）预处理的影响。得出的结论是，“致密囊泡”酶在胰岛素的这一作用中不发挥重要作用，并且尚未确定的环磷酸腺苷磷酸二酯酶必定被胰岛素激活。用氯化铵或果糖处理肝细胞似乎会可逆地增加环磷酸腺苷磷酸二酯酶活性。当用N6 -（苯异丙基）腺苷来防止胰高血糖素阻断胰岛素对质膜环磷酸腺苷磷酸二酯酶活性的激活作用时，胰岛素降低胰高血糖素处理的肝细胞中细胞内环磷酸腺苷浓度的能力显著增强。胰岛素对质膜环磷酸腺苷磷酸二酯酶活性的激活作用在降低由胰高血糖素升高的细胞内环磷酸腺苷浓度方面可发挥强大作用。
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An assessment of the ability of insulin-stimulated cyclic AMP phosphodiesterase to decrease hepatocyte intracellular cyclic AMP concentrations.对胰岛素刺激的环磷酸腺苷磷酸二酯酶降低肝细胞内循环磷酸腺苷浓度能力的评估。
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引用本文的文献
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2
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本文引用的文献
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Challenge of hepatocytes by glucagon triggers a rapid modulation of adenylate cyclase activity in isolated membranes.胰高血糖素对肝细胞的刺激会引发分离膜中腺苷酸环化酶活性的快速调节。
Biochem J. 1983 Jul 15;214(1):93-8. doi: 10.1042/bj2140093.
2
Insulin controls intracellular cyclic AMP concentrations in hepatocytes by activating specific cyclic AMP phosphodiesterases: phosphorylation of the peripheral plasma membrane enzyme.胰岛素通过激活特定的环磷酸腺苷磷酸二酯酶来控制肝细胞内的环磷酸腺苷浓度：外周质膜酶的磷酸化。
Adv Cyclic Nucleotide Protein Phosphorylation Res. 1984;16:159-76.
3
Insulin activates the plasma-membrane and dense-vesicle cyclic AMP phosphodiesterase in hepatocytes by distinct routes.胰岛素通过不同途径激活肝细胞中的质膜和致密小泡环磷酸腺苷磷酸二酯酶。
Biochem J. 1983 Oct 15;216(1):245-8. doi: 10.1042/bj2160245.
4
Insulin exerts actions through a distinct species of guanine nucleotide regulatory protein: inhibition of adenylate cyclase.胰岛素通过一种独特的鸟嘌呤核苷酸调节蛋白发挥作用：抑制腺苷酸环化酶。
Biochem J. 1983 Aug 15;214(2):547-52. doi: 10.1042/bj2140547.
5
Insulin and glucagon regulate the activation of two distinct membrane-bound cyclic AMP phosphodiesterases in hepatocytes.胰岛素和胰高血糖素调节肝细胞中两种不同的膜结合环磷酸腺苷磷酸二酯酶的活性。
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Guanine nucleotides can activate the insulin-stimulated phosphodiesterase in liver plasma membranes.鸟嘌呤核苷酸可激活肝细胞膜中受胰岛素刺激的磷酸二酯酶。
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Characterization of the phosphorylated form of the insulin-stimulated cyclic AMP phosphodiesterase from rat liver plasma membranes.大鼠肝细胞膜中胰岛素刺激的环磷酸腺苷磷酸二酯酶磷酸化形式的特性研究
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