非洲爪蟾和小鼠线粒体基因组的共线性组织

Co-linear organization of Xenopus laevis and mouse mitochondrial genomes.

作者信息

Champagne A M, Dennebouy N, Julien J F, Lehegarat J C, Mounolou J C

出版信息

Biochem Biophys Res Commun. 1984 Aug 16;122(3):918-24. doi: 10.1016/0006-291x(84)91178-1.

Abstract

Cloned fragments of Xenopus laevis mitochondrial DNA and Pleurodeles waltlii mitochondrial cDNAs have been hybridized together and with mouse mtDNA. In the three cases cross-hybridization was observed. The overall organization of the X. laevis fragments appeared to be co-linear with the mouse mtDNA, most sequences being conserved except for the D-loop and the URF6 regions. The use of mouse mtDNA has enabled us to identified several mitochondrial genes in X. laevis and P. waltlii.

摘要

非洲爪蟾线粒体DNA的克隆片段与美西螈线粒体cDNA已相互杂交，并与小鼠线粒体DNA进行了杂交。在这三种情况下均观察到了交叉杂交现象。非洲爪蟾片段的总体结构似乎与小鼠线粒体DNA呈共线性，除了D环和URF6区域外，大多数序列是保守的。使用小鼠线粒体DNA使我们能够在非洲爪蟾和美西螈中鉴定出几个线粒体基因。