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一种与单链DNA结合的线粒体蛋白的特性分析。

Characterization of a mitochondrial protein binding to single-stranded DNA.

作者信息

Mignotte B, Barat M, Mounolou J C

出版信息

Nucleic Acids Res. 1985 Mar 11;13(5):1703-16. doi: 10.1093/nar/13.5.1703.

Abstract

A DNA-binding protein from Xenopus laevis oocyte mitochondria which has been found associated with the D-loop also shows a strong preference for single-stranded DNA. The binding to polynucleotides is dependent on the base composition, but no sequence specificity was found. This protein, called mtSSB, binds tightly and cooperatively to single-stranded DNA. By its amino-acid composition and its binding properties it appears to be similar to the single-stranded DNA-binding proteins found in prokaryotes.

摘要

在非洲爪蟾卵母细胞线粒体中发现的一种与D环相关的DNA结合蛋白,也表现出对单链DNA的强烈偏好。与多核苷酸的结合取决于碱基组成,但未发现序列特异性。这种名为mtSSB的蛋白与单链DNA紧密且协同结合。从其氨基酸组成和结合特性来看,它似乎与原核生物中发现的单链DNA结合蛋白相似。

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