Hadjian A J, Culty M, Chambaz E M
Biochem Biophys Res Commun. 1984 Aug 30;123(1):33-40. doi: 10.1016/0006-291x(84)90376-0.
Phospholipase C (Bacillus cereus) added to the incubation medium stimulated the steroidogenic activity of bovine adrenal zona fasciculata cell suspensions to a level similar to that induced by optimal concentration of ACTH. This effect was not related to an increase of cyclic AMP; it was calcium-dependent and was also induced by an other bacterial phospholipase C (from Clostridium perfringens) whereas phospholipases A2 and D were ineffective. Phospholipid metabolism was examined in these cells after radiolabeling with [14C]-glycerol or [32P]orthophosphate. Phospholipase C induced a very fast (5 seconds) increase in cellular [14C]-1,2-diacylglycerol followed by [32P] labeling of phosphatidic acid and phosphatidylinositol. These events preceded the stimulation of steroidogenesis which was detectable after 2 minutes of incubation. These observations suggest that activation of an endogenous phospholipase C activity may be considered as an early event in the response of bovine adrenocortical cells to steroidogenic effectors such as angiotensin II and acetylcholine.
添加到孵育培养基中的磷脂酶C（蜡样芽孢杆菌）可将牛肾上腺束状带细胞悬液的类固醇生成活性刺激到与促肾上腺皮质激素最佳浓度所诱导的水平相似。这种作用与环磷酸腺苷的增加无关；它依赖于钙，并且也可由另一种细菌磷脂酶C（产气荚膜梭菌）诱导，而磷脂酶A2和D则无效。在用[14C] -甘油或[32P]正磷酸盐进行放射性标记后，对这些细胞中的磷脂代谢进行了检查。磷脂酶C诱导细胞内[14C] -1,2 -二酰基甘油非常快速（5秒）地增加，随后是磷脂酸和磷脂酰肌醇的[32P]标记。这些事件先于类固醇生成的刺激，后者在孵育2分钟后即可检测到。这些观察结果表明，内源性磷脂酶C活性的激活可能被视为牛肾上腺皮质细胞对诸如血管紧张素II和乙酰胆碱等类固醇生成效应物反应中的早期事件。
